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Data-driven computational protein design
(Elsevier BV, 2021-08)
Computational protein design can generate proteins not found in nature that adopt desired structures and perform novel functions. Although proteins could, in theory, be designed with ab initio methods, practical success ...
A validated single-cell-based strategy to identify diagnostic and therapeutic targets in complex diseases
(Springer Science+Business Media, 2019-07-30)
Background: Genomic medicine has paved the way for identifying biomarkers and therapeutically actionable targets for complex diseases, but is complicated by the involvement of thousands of variably expressed genes across ...
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
(Springer Nature, 2018-01-29)
CRISPR-Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This ...
The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis
(Elsevier BV, 2019-07)
Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots, "Huang Qin," are used for liver and lung complaints and as complementary cancer treatments. We report a ...
Casting a wider net: Immunosurveillance by nonclassical MHC molecules
(Public Library of Science (PLoS), 2019-02-21)
Most studies of T lymphocytes focus on recognition of classical major histocompatibility complex (MHC) class I or II molecules presenting oligopeptides, yet there are numerous variations and exceptions of biological ...
Pièce de Self-Résistance: a New Paradigm for Natural-Product Herbicide Discovery
(Elsevier BV, 2018-09)
Modern agriculture is faced with the challenge of feeding a rapidly growing population.While crop yields have risen dramatically since the beginning of the twentieth century, increasing demand for foodthreatens to outstrip ...
Identification of the minimal bacterial 2′-deoxy-7-amido-7-deazaguanine synthesis machinery
(Wiley, 2018-10-12)
The 7-deazapurine derivatives, 2′-deoxy-7-cyano-7-deazaguanosine (dPreQ0) and 2′-deoxy-7-amido-7-deazaguanosine (dADG) are recently discovered DNA modifications encoded by the dpd cluster found in a diverse set of bacteria. ...
Multi-cell ECM compaction is predictable via superposition of nonlinear cell dynamics linearized in augmented state space
(Public Library of Science (PLoS), 2019-09-20)
Cells interacting through an extracellular matrix (ECM) exhibit emergent behaviors resulting from collective intercellular interaction. In wound healing and tissue development, characteristic compaction of ECM gel is induced ...
Structural and Functional Analysis of E. coli Cyclopropane Fatty Acid Synthase
(Elsevier BV, 2018-09)
Cell membranes must adapt to different environments. In Gram-negative bacteria, the inner membrane can be remodeled directly by modification of lipids embedded in the bilayer. For example, when Escherichia coli enters ...
Dynamic filopodial forces induce accumulation, damage, and plastic remodeling of 3D extracellular matrices
(Public Library of Science (PLoS), 2019-04-08)
The mechanical properties of the extracellular matrix (ECM)–a complex, 3D, fibrillar scaffold of cells in physiological environments–modulate cell behavior and can drive tissue morphogenesis, regeneration, and disease ...