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A validated single-cell-based strategy to identify diagnostic and therapeutic targets in complex diseases
(Springer Science+Business Media, 2019-07-30)
Background: Genomic medicine has paved the way for identifying biomarkers and therapeutically actionable targets for complex diseases, but is complicated by the involvement of thousands of variably expressed genes across ...
Collagen Prolyl Hydroxylases Are Bifunctional Growth Regulators in Melanoma
(Elsevier Science Ltd., 2018-11-16)
Appropriate post-translational processing of collagen requires prolyl hydroxylation, catalyzed by collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase, and is essential for normal cell function. Here we have ...
Identification of cell cycle-targeting microRNAs through genome-wide screens
(Informa UK Limited, 2017-11-14)
By performing nine genome-wide microRNA (miRNA) screens, we recently uncovered a new class of miRNAs, which target multiple cyclins and cyclin-dependent kinases (CDKs). Systemic delivery of selected cell cycle-targeting ...
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
(Springer Nature, 2018-01-29)
CRISPR-Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This ...
Structural and Functional Analysis of E. coli Cyclopropane Fatty Acid Synthase
(Elsevier BV, 2018-09)
Cell membranes must adapt to different environments. In Gram-negative bacteria, the inner membrane can be remodeled directly by modification of lipids embedded in the bilayer. For example, when Escherichia coli enters ...
The Human Lung Cell Atlas: A High-Resolution Reference Map of the Human Lung in Health and Disease
(American Thoracic Society, 2019-07)
Lung disease accounts for every sixth death globally. Profiling the molecular state of all lung cell types in health and disease is currently revolutionizing the identification of disease mechanisms and will aid the design ...
Small and Large Ribosomal Subunit Deficiencies Lead to Distinct Gene Expression Signatures that Reflect Cellular Growth Rate
(Elsevier BV, 2019-01)
In this issue of Molecular Cell, Cheng et al. report specific and dose-dependent gene expression changes resulting from decreased ribosome levels, including distinct gene expression signatures resulting from loss of genes ...
Spatial Exclusivity Combined with Positive and Negative Selection of Phosphorylation Motifs Is the Basis for Context-Dependent Mitotic Signaling
(American Association for the Advancement of Science (AAAS), 2011-06-28)
The timing and localization of events during mitosis are controlled by the regulated phosphorylation of proteins by the mitotic kinases, which include Aurora A, Aurora B, Nek2 (never in mitosis kinase 2), Plk1 (Polo-like ...
Histidine N(τ)-cyclized macrocycles as a new genre of polo-like kinase 1 polo-box domain-binding inhibitors
(Elsevier BV, 2018-10)
Transition toward peptide mimetics of reduced size is an important objective of peptide macrocyclization. We have previously shown that PLH∗SpT (2a) (where H∗ indicates the presence of a –(CH2)8Ph group at the N(π) position ...
The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis
(Elsevier BV, 2019-07)
Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots, "Huang Qin," are used for liver and lung complaints and as complementary cancer treatments. We report a ...