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Genetic and molecular identification of three human TPP1 functions in telomerase action: recruitment, activation, and homeostasis set point regulation
(Cold Spring Harbor Laboratory Press, 2014-08)
Telomere length homeostasis is essential for the long-term survival of stem cells, and its set point determines the proliferative capacity of differentiated cell lineages by restricting the reservoir of telomeric repeats. ...
Human Naive Pluripotent Stem Cells Model X Chromosome Dampening and X Inactivation
(Elsevier, 2016-12)
Naive human embryonic stem cells (hESCs) can be derived from primed hESCs or directly from blastocysts, but their X chromosome state has remained unresolved. Here, we show that the inactive X chromosome (Xi) of primed hESCs ...
The Developmental Potential of iPSCs Is Greatly Influenced by Reprogramming Factor Selection
(Elsevier, 2014-09)
Induced pluripotent stem cells (iPSCs) are commonly generated by transduction of Oct4, Sox2, Klf4, and Myc (OSKM) into cells. Although iPSCs are pluripotent, they frequently exhibit high variation in terms of quality, as ...
Mechanisms and models of somatic cell reprogramming
(Nature Publishing Group, 2013-05)
Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions
(National Academy of Sciences, 2011-11)
The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical ...
Chromatin Structure and Gene Expression Programs of Human Embryonic and Induced Pluripotent Stem Cells
(Elsevier, 2010-08)
Knowledge of both the global chromatin structure and the gene expression programs of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) should provide a robust means to assess whether the genomes ...
X-linked H3K27me3 demethylase Utx is required for embryonic development in a sex-specific manner
(National Academy of Sciences (U.S.), 2012-07)
Embryogenesis requires the timely and coordinated activation of developmental regulators. It has been suggested that the recently discovered class of histone demethylases (UTX and JMJD3) that specifically target the ...
Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors
(Elsevier, 2012-09)
Sertoli cells are considered the “supporting cells” of the testis that play an essential role in sex determination during embryogenesis and in spermatogenesis during adulthood. Their essential roles in male fertility along ...
Direct Lineage Conversion of Adult Mouse Liver Cells and B Lymphocytes to Neural Stem Cells
(Elsevier, 2014-11)
Overexpression of transcription factors has been used to directly reprogram somatic cells into a range of other differentiated cell types, including multipotent neural stem cells (NSCs), that can be used to generate neurons ...
Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene
(Elsevier BV, 2018-02)
Fragile X syndrome (FXS), the most common genetic form of intellectual disability in males, is caused by silencing of the FMR1 gene associated with hypermethylation of the CGG expansion mutation in the 5′ UTR of FMR1 in ...