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CXCR4-expressing Mist1+ progenitors in the gastric antrum contribute to gastric cancer development
(Impact Journals, LLC, 2017-11)
Mist1 was recently shown to identify a discrete population of stem cells within the isthmus of the oxyntic gland within the gastric corpus. Chief cells at the base of the gastric corpus also express Mist1. The relevance ...
Differential selective pressure alters rate of drug resistance acquisition in heterogeneous tumor populations
(Nature Publishing Group, 2016-11)
Recent drug discovery and development efforts have created a large arsenal of targeted and chemotherapeutic drugs for precision medicine. However, drug resistance remains a major challenge as minor pre-existing resistant ...
Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
(Public Library of Science, 2011-11)
A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. ...
Direct visualization of DNA affinity landscapes using a highthroughput sequencing instrument
(Nature Publishing Group, 2011-06)
Several methods for characterizing DNA-protein interactions are available, but none have demonstrated both high throughput and quantitative measurement of affinity. Here we describe 'high-throughput sequencing'-'fluorescent ...
Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins
(BioMed Central Ltd, 2006-03)
Background: Kinetochores are large multi-protein structures that assemble on centromeric DNA (CEN DNA) and mediate the binding of chromosomes to microtubules. Comprising 125 base-pairs of CEN DNA and 70 or more protein ...
In vivo measurements of interindividual differences in DNA glycosylases and APE1 activities
(National Academy of Sciences (U.S.), 2017-11)
The integrity of our DNA is challenged with at least 100,000 lesions per cell on a daily basis. Failure to repair DNA damage efficiently can lead to cancer, immunodeficiency, and neurodegenerative disease. Base excision ...
A requirement for filopodia extension toward Slit during Robo-mediated axon repulsion
(Rockefeller University Press, 2016-04)
Axons navigate long distances through complex 3D environments to interconnect the nervous system during development. Although the precise spatiotemporal effects of most axon guidance cues remain poorly characterized, a ...
Combinatorial bZIP dimers display complex DNA-binding specificity landscapes
(eLife Sciences Publications, Ltd., 2017-02)
How transcription factor dimerization impacts DNA-binding specificity is poorly understood. Guided by protein dimerization properties, we examined DNA binding specificities of 270 human bZIP pairs. DNA interactomes of 80 ...
Direct Identification of Hundreds of Expression-Modulating Variants using a Multiplexed Reporter Assay
(Elsevier, 2018-02)
Although studies have identified hundreds of loci associated with human traits and diseases, pinpointing causal alleles remains difficult, particularly for non-coding variants. To address this challenge, we adapted the ...
A Fungal-Selective Cytochrome bc₁ Inhibitor Impairs Virulence and Prevents the Evolution of Drug Resistance
(Elsevier, 2016-08)
To cause disease, a microbial pathogen must adapt to the challenges of its host environment. The leading fungal pathogen Candida albicans colonizes nutrient-poor bodily niches, withstands attack from the immune system, and ...