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dc.contributor.authorSahai, Erik
dc.contributor.authorWyckoff, Jeffrey
dc.contributor.authorPhilippar, Ulrike
dc.contributor.authorSegall, Jeffrey E
dc.contributor.authorGertler, Frank
dc.contributor.authorCondeelis, John S.
dc.date.accessioned2010-09-23T12:59:02Z
dc.date.available2010-09-23T12:59:02Z
dc.date.issued2005-05
dc.date.submitted2005-02
dc.identifier.issn1472-6750
dc.identifier.urihttp://hdl.handle.net/1721.1/58675
dc.description.abstractBackground: The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy. Results: Here we describe a method for the simultaneous imaging, by multiphoton microscopy, of Green Fluorescent Protein, Cyan Fluorescent Protein and collagen in vivo in living tumors. This novel method enables: 1) the simultaneous visualization of overall cell shape and sub-cellular structures such as the plasma membrane or proteins of interest in cells inside living animals, 2) direct comparison of the behavior of single cells from different cell lines in the same microenvironment in vivo. Conclusion: Using this multi-fluor, multiphoton technique, we demonstrate that motility and metastatic differences between carcinoma cells of differing metastatic potential can be imaged in the same animal simultaneously at sub-cellular resolution.en_US
dc.description.sponsorshipNational Institutes of Health (U.S) ( grant CA100324)en_US
dc.description.sponsorshipNational Institutes of Health (U.S) ( grant GM58801)en_US
dc.description.sponsorshipInternational Union against Cancer (Aventis Translational Cancer Research Fellowship)en_US
dc.publisherBioMed Central Ltden_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/1472-6750-5-14en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_US
dc.sourceBioMed Central Ltden_US
dc.titleSimultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopyen_US
dc.typeArticleen_US
dc.identifier.citationBMC Biotechnology. 2005 May 23;5(1):14en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorPhilippar, Ulrike
dc.contributor.mitauthorGertler, Frank
dc.relation.journalBMC Biotechnologyen_US
dc.eprint.versionFinal published versionen_US
dc.identifier.pmid15910685
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2010-09-03T16:23:21Z
dc.language.rfc3066en
dc.rights.holderSahai et al.; licensee BioMed Central Ltd.
dspace.orderedauthorsSahai, Erik; Wyckoff, Jeffrey; Philippar, Ulrike; Segall, Jeffrey E; Gertler, Frank; Condeelis, Johnen
dc.identifier.orcidhttps://orcid.org/0000-0003-3214-4554
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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