Validation and refinement of gene-regulatory pathways on a network of physical interactions
Author(s)Yeang, Chen-Hsiang, 1969-; Mak, Craig; McCuine, Scott; Workman, Christopher; Ideker, Trey; Jaakkola, Tommi S.; ... Show more Show less
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As genome-scale measurements lead to increasingly complex models of gene regulation, systematic approaches are needed to validate and refine these models. Towards this goal, we describe an automated procedure for prioritizing genetic perturbations in order to discriminate optimally between alternative models of a gene-regulatory network. Using this procedure, we evaluate 38 candidate regulatory networks in yeast and perform four high-priority gene knockout experiments. The refined networks support previously unknown regulatory mechanisms downstream of SOK2 and SWI4.
DepartmentMassachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
BioMed Central Ltd
Genome Biology. 2005 Jul 01;6(7):R62
Final published version