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dc.contributor.authorAngelosanto, Jill
dc.contributor.authorBrosnahan, Kathleen
dc.contributor.authorRoss, Kenneth
dc.contributor.authorHahn, Cynthia
dc.contributor.authorRussell, Kate
dc.contributor.authorDrury, Linda
dc.contributor.authorNorton, Stephanie
dc.contributor.authorNadler, Lee
dc.contributor.authorStegmaier, Kimberly
dc.contributor.authorHaining, W. Nicholas
dc.date.accessioned2010-10-13T15:17:50Z
dc.date.available2010-10-13T15:17:50Z
dc.date.issued2008-08
dc.date.submitted2008-05
dc.identifier.issn1471-2172
dc.identifier.urihttp://hdl.handle.net/1721.1/59279
dc.description.abstractBackground: The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells. Results: Here, we describe a method for gene-expression based screening that allows primary naive and memory-phenotype lymphocytes to be discriminated based on complex genes signatures corresponding to these differentiation states. We used ligation-mediated amplification and a fluorescent, bead-based detection system to quantify simultaneously 55 transcripts representing naive and memory-phenotype signatures in purified populations of human T cells. The use of a multi-gene panel allowed better resolution than any constituent single gene. The method was precise, correlated well with Affymetrix microarray data, and could be easily scaled up for high-throughput. Conclusion: This method provides a generic solution for high-throughput differentiation screens in primary human T cells where no single-gene or functional assay is available. This screening platform will allow the identification of small molecules, genes or soluble factors that direct memory differentiation in naive human lymphocytes.en_US
dc.publisherBioMed Central Ltden_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/1471-2172-9-44en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_US
dc.sourceBioMed Central Ltden_US
dc.titleHigh-throughput gene expression profiling of memory differentiation in primary human T cellsen_US
dc.typeArticleen_US
dc.identifier.citationBMC Immunology. 2008 Aug 01;9(1):44en_US
dc.contributor.departmentBroad Institute of MIT and Harvarden_US
dc.contributor.mitauthorRoss, Kenneth
dc.contributor.mitauthorNorton, Stephanie
dc.contributor.mitauthorStegmaier, Kimberly
dc.relation.journalBMC Immunologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2010-09-03T16:13:43Z
dc.language.rfc3066en
dc.rights.holderHaining et al.; licensee BioMed Central Ltd.
dspace.orderedauthorsHaining, W. N.; Angelosanto, Jill; Brosnahan, Kathleen; Ross, Kenneth; Hahn, Cynthia; Russell, Kate; Drury, Linda; Norton, Stephanie; Nadler, Lee; Stegmaier, Kimberlyen
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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