Fast scanning two-photon microscopy

Author(s)
Chang, Jeremy T
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Alternative title
Fast scanning 2-photon microscopy
Fast scanning two-photon microscope
Other Contributors
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Advisor
Edward S. Boyden.
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Abstract
Fast scanning two-photon microscopy coupled with the use light activated ion channels provides the basis for fast imaging and stimulation in the characterization of in vivo neural networks. A two-photon microscope capable of fast scanning using acousto-optic deflectors was designed and implemented. The software controller was expanded so that random access scan in three dimensions could be handled, so that algorithms that can identify neurons from images acquired using the two-photon microscope can be developed. Finally the localization of optogenetic Channelrhodopsin-2 channel to the neuron cell body was tested using a ChR2-MBD construct.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2010.
 
Cataloged from PDF version of thesis.
 
Includes bibliographical references (p. 45-46).
 
Date issued
2010
URI
http://hdl.handle.net/1721.1/61148
Department
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Publisher
Massachusetts Institute of Technology
Keywords
Electrical Engineering and Computer Science.
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