Manipulation of bacteria using three dimensional insulator based dielectrophoresis
Author(s)Braff, William Allan
Manipulation of bacteria using 3 dimensional insulator based dielectrophoresis
Manipulation of bacteria using 3D insulator based dielectrophoresis
Manipulation of bacteria using 3DiDEP
Manipulation of bacteria using three dimensional iDEP
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Cullen R. Buie.
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Insulator-based dielectrophoresis (iDEP) is a very promising technique for sorting microparticles based on their electrical properties. By using constrictions in a microchannel to generate large electric field gradients, the need for microfabricated electrode arrays is eliminated. In this work, three-dimensional insulator-based dielectrophoresis (3DiDEP) devices are fabricated using a novel micromachining technique. These devices are predicted and observed to exhibit higher trapping sensitivity than existing devices in the literature; this allows for efficient trapping of microparticles and bacteria at low applied fields with minimal thermal shock on the particles due to Joule heating effects. The dielectric properties of a range of bacteria, as well as polystyrene beads are examined. The impact of these results on the development of microbial fuel cells and portable low-cost clinical diagnostic tools is also discussed.
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2011.Cataloged from PDF version of thesis.Includes bibliographical references (p. 103-111).
DepartmentMassachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology