The role of mir-290-295 in murine embryonic and germ cell development
Author(s)Medeiros, Lea A. (Lea Ann)
Massachusetts Institute of Technology. Dept. of Biology.
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MicroRNAs, 22nt long RNAs derived from hairpin transcripts, are important regulators of gene expression and have been shown to participate in regulation of every biological process known to date. Mir-290 through mir-295 (mir-290-295) is a mammalian-specific miRNA cluster that, in the mouse, is expressed specifically in early embryos and embryonic germ cells. This thesis examines the in vivo consequences of targeted deletion of mir-290-295 in the developing mouse embryo. Mir-290-295 deficiency results in a partially penetrant embryonic lethality. Mir-290-295 is not required for early preimplantation development but is instead required for later postimplantation development. In surviving mir-290-295 deficient embryos, female but not male fertility is compromised. This impairment in fertility arises from a defect in migrating primordial germ cells and occurs equally in male and female mutant animals. Male mir-290-295-- mice, due to the extended proliferative lifespan of their germ cells, are able to recover from this initial germ cell loss and are fertile. Female mir-290-295-/- mice are unable to recover and are sterile due to premature ovarian failure.
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, September 2011.Cataloged from PDF version of thesis. "September 2011."Includes bibliographical references.
DepartmentMassachusetts Institute of Technology. Dept. of Biology.
Massachusetts Institute of Technology