Show simple item record

dc.contributor.advisorJoseph Jacobson.en_US
dc.contributor.authorSterling, Bram (Bram H.)en_US
dc.contributor.otherMassachusetts Institute of Technology. Dept. of Architecture. Program in Media Arts and Sciences.en_US
dc.date.accessioned2012-03-16T16:04:58Z
dc.date.available2012-03-16T16:04:58Z
dc.date.copyright2011en_US
dc.date.issued2011en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/69809
dc.descriptionThesis (S.M.)--Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2011.en_US
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references (p. 28).en_US
dc.description.abstractIn order to modify bacterial genetic codes, modifications must be made throughout the genome wherever the affected codon is used. Making such numerous and widespread genomic changes requires specialized techniques. MAGE is well-suited to this task, being highly amenable to multiplexing and having low time and resource costs per site. MAGE has been used as a first stage in recoding efforts, converting small clusters of sites in separate strains to be combined by other means, but improvements in MAGE technique suggest the possibility of using it to produce fully-recoded strains directly. To this end, I compare strategies based on co-selected MAGE and apply the best by performing 80 site conversions spread over 1/4 of the E coli genome.en_US
dc.description.statementofresponsibilityby Bram Sterling.en_US
dc.format.extent32 p.en_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsM.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectArchitecture. Program in Media Arts and Sciences.en_US
dc.titleExploration of methods for many-site genome editing with MAGEen_US
dc.typeThesisen_US
dc.description.degreeS.M.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Dept. of Architecture. Program in Media Arts and Sciences.en_US
dc.identifier.oclc778070056en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record