Raman Spectroscopy Provides a Powerful Diagnostic Tool for Accurate Determination of Albumin Glycation
Author(s)
Dingari, Narahara Chari; Horowitz, Gary L.; Kang, Jeon Woong; Dasari, Ramachandra Rao; Barman, Ishan
DownloadDingari-2012-Raman spectroscopy p.pdf (629.1Kb)
PUBLISHER_CC
Publisher with Creative Commons License
Creative Commons Attribution
Terms of use
Metadata
Show full item recordAbstract
We present the first demonstration of glycated albumin detection and quantification using Raman spectroscopy without the addition of reagents. Glycated albumin is an important marker for monitoring the long-term glycemic history of diabetics, especially as its concentrations, in contrast to glycated hemoglobin levels, are unaffected by changes in erythrocyte life times. Clinically, glycated albumin concentrations show a strong correlation with the development of serious diabetes complications including nephropathy and retinopathy. In this article, we propose and evaluate the efficacy of Raman spectroscopy for determination of this important analyte. By utilizing the pre-concentration obtained through drop-coating deposition, we show that glycation of albumin leads to subtle, but consistent, changes in vibrational features, which with the help of multivariate classification techniques can be used to discriminate glycated albumin from the unglycated variant with 100% accuracy. Moreover, we demonstrate that the calibration model developed on the glycated albumin spectral dataset shows high predictive power, even at substantially lower concentrations than those typically encountered in clinical practice. In fact, the limit of detection for glycated albumin measurements is calculated to be approximately four times lower than its minimum physiological concentration. Importantly, in relation to the existing detection methods for glycated albumin, the proposed method is also completely reagent-free, requires barely any sample preparation and has the potential for simultaneous determination of glycated hemoglobin levels as well. Given these key advantages, we believe that the proposed approach can provide a uniquely powerful tool for quantification of glycation status of proteins in biopharmaceutical development as well as for glycemic marker determination in routine clinical diagnostics in the future.
Date issued
2012-02Department
Massachusetts Institute of Technology. Laser Biomedical Research Center; Massachusetts Institute of Technology. Spectroscopy LaboratoryJournal
PLoS ONE
Publisher
Public Library of Science
Citation
Dingari, Narahara Chari et al. “Raman Spectroscopy Provides a Powerful Diagnostic Tool for Accurate Determination of Albumin Glycation.” Ed. Irene Georgakoudi. PLoS ONE 7.2 (2012): e32406. Web.
Version: Final published version
ISSN
1932-6203