| dc.contributor.advisor | Michael Strano. | en_US |
| dc.contributor.author | Bojö, Peter | en_US |
| dc.contributor.other | Massachusetts Institute of Technology. Dept. of Biological Engineering. | en_US |
| dc.date.accessioned | 2012-07-02T15:43:14Z | |
| dc.date.available | 2012-07-02T15:43:14Z | |
| dc.date.copyright | 2011 | en_US |
| dc.date.issued | 2012 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1721.1/71463 | |
| dc.description | Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, February 2012. | en_US |
| dc.description | Cataloged from PDF version of thesis. | en_US |
| dc.description | Includes bibliographical references (p. 46-51). | en_US |
| dc.description.abstract | This work investigated single-walled carbon nanotube (SWNT)/polymer-protein A complexes for optically reporting antibody concentration via a change in near infrared fluorescent emission after antibody binding. SWNT have potential as biosensors because of extraordinary sensitivity, lack of photobleaching, and optical activity in a near-infrared window. A SWNT sensor could provide label-free measurements of antibody concentration in a continuous fashion, which may aid selection of production strains. Protein A itself, dextran, poly vinyl alcohol, DNA sequences, and chitosan were used as polymers for wrapping SWNT. Nonspecific binding to solution-phase constructs was found to be a major problem with these approaches. Chitosan hydrogels encapsulating SWNT also show nonspecific responses. | en_US |
| dc.description.statementofresponsibility | by Peter Bojö. | en_US |
| dc.format.extent | 51 p. | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Massachusetts Institute of Technology | en_US |
| dc.rights | MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided. | en_US |
| dc.rights.uri | http://dspace.mit.edu/handle/1721.1/7582 | en_US |
| dc.subject | Biological Engineering. | en_US |
| dc.title | Towards a carbon nanotube antibody sensor | en_US |
| dc.type | Thesis | en_US |
| dc.description.degree | M.Eng. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | |
| dc.identifier.oclc | 795184338 | en_US |
