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dc.contributor.advisorEdward F. De long.en_US
dc.contributor.authorBuck, Justin Daviden_US
dc.contributor.otherMassachusetts Institute of Technology. Dept. of Biological Engineering.en_US
dc.date.accessioned2012-07-02T15:43:19Z
dc.date.available2012-07-02T15:43:19Z
dc.date.copyright2012en_US
dc.date.issued2012en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/71464
dc.descriptionThesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2012.en_US
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references (p. 175-195).en_US
dc.description.abstractProteorhodopsin (PR) phototrophy plays an important role in the marine ecosystem, harvesting energy from sunlight for a diverse community of hetertrophic organisms. The simple proteorhodopsin photosystem (PRPS) composed of six to seven genes is sufficient for producing a functional light-driven proton pump, capable of powering cellular processes. This thesis describes the functional characterization of a subcloned PRPS previously identified from a large insert metagenomic library (Martinez et al., 2007). Incorporation of the PRPS into a strain of Pseudomonas putida resulted in a light-dependent increase in viable cell yield of cultures grown in low carbon media. The light-dependent effect demonstrates a dependence on carbon, reducing at increasing carbon concentrations until no differential effect is observed. A survey of six PR-containing vectors from metagenomic libraries revealed PR transcription in two hosts, P. putida and Pseudoalteromonas atlantica, and of the three additional vectors with PRPS tested, two demonstrated the same qualitative light-dependent yield increase. This work illustrates the utility of a simple rhodopsin photosystem for supplementing the cellular energy system of a heterologous host, paving the way for future engineering applications in photoheterotrophy.en_US
dc.description.statementofresponsibilityby Justin David Buck.en_US
dc.format.extent195 p.en_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsMIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectBiological Engineering.en_US
dc.titlePhysiological effects of heterologous expression of proteorhodopsin photosystemsen_US
dc.typeThesisen_US
dc.description.degreePh.D.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Dept. of Biological Engineering.en_US
dc.identifier.oclc795184381en_US


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