Embryonic stem cell-based mapping of developmental transcriptional programs
Author(s)Mazzoni, Esteban O.; Mahony, Shaun; Iacovino, Michelina; Morrison, Carolyn A.; Mountoufaris, George; Closser, Michael; Whyte, Warren Anthony; Young, Richard A.; Kyba, Michael; Gifford, David K.; Wichterle, Hynek; ... Show more Show less
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The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.
DepartmentMassachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Whitehead Institute for Biomedical Research
Nature Publishing Group
Mazzoni, Esteban O et al. “Embryonic Stem Cell–based Mapping of Developmental Transcriptional Programs.” Nature Methods 8.12 (2011): 1056–1058.
Author's final manuscript