Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator
Author(s)Chen, Chia-Hung; Sarkar, Aniruddh; Song, Yong-Ak; Miller, Miles Aaron; Kim, Sung Jae; Griffith, Linda G.; Lauffenburger, Douglas A.; Han, Jongyoon; ... Show more Show less
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We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Journal of the American Chemical Society
American Chemical Society (ACS)
Chen, Chia-Hung et al. “Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.” Journal of the American Chemical Society 133.27 (2011): 10368–10371.
Author's final manuscript