Browsing Biological Engineering - Master's degree by Title
Now showing items 65-84 of 95
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Optimization of cell adhesion environments for a liver cell bioreactor
(Massachusetts Institute of Technology, 2006)The MilliF bioreactor offers great potential for the formation of i vivo-like liver tissue outside the body, making it a valuable tool for applications such as drug toxicity models and biosensors. Cell adhesion is an ... -
Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
(Massachusetts Institute of Technology, 2006)Nonviral vector research and development has been stunted by a lack of knowledge and understanding of how vectors are trafficked within the cell. Research currently involves mass screenings of different combinations of ... -
Process Improvement in Biotech : Dealing with High Complexity Processes
(Massachusetts Institute of Technology, 2009)Across numerous conventional manufacturing sites, process improvement initiatives have been shown to increase production capabilities while decreasing costs - all without a required system- wide overhaul of the manufacturing ... -
Quantitation of 8-oxoG and strand breaks in pUC19 plasmid DNA after exposure to oxygen radical generating systems
(Massachusetts Institute of Technology, 1995) -
Quantitative analysis of TLR-4-mediated cell responses in murine macrophages
(Massachusetts Institute of Technology, 2008)TLR-4 is essential in host defense against bacterial infection. By recognition of specific pathogen-associated molecular patterns such as lipopolysaccharide (LPS), TLR-4 can in tandem initiate a pair of downstream signaling ... -
A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage
(Massachusetts Institute of Technology, 2011)Objectives: 1) To perform a quantitative comparison of proteins released to media on combination with cytokine (IL-1[beta[ or TNF-[alpha]) and Injury as compared to either treatment alone, and to thus identify proteins ... -
A rapid, flexible and scalable DNA assembly platform for genome engineering and regulated gene expression applications in Plasmodium falciparum
(Massachusetts Institute of Technology, 2015)Plasmodium falciparum is the deadliest malaria parasite. There is no approved vaccine to prevent this disease, and resistance to available antimalarial drugs is becoming widespread. Identification of parasite genes essential ... -
Regulation of apoptosis in human cancer cells
(Massachusetts Institute of Technology, 2005)Nitric oxide is postulated to protect cancer cells from the death-inducing effects of tumour necrosis factor alpha by S-nitrosating the active site cysteines, inhibiting cleavage of caspase-9. We aimed to test this hypothesis ... -
Regulation of jun B gene expression in v-fos tranformed rat-1 fibroblasts and revertants
(Massachusetts Institute of Technology, 1994) -
Regulation of specific connexins differentially alters gap junction permeability and endothelial cell function
(Massachusetts Institute of Technology, 2006)While many have explored how vascular processes alter gap junction communication and composition few have analyzed the role of specific gap junction connexin proteins in regulating cellular communication and wound healing. ... -
The role of polyphosphate kinase in long term survival of Helicobacter pylori
(Massachusetts Institute of Technology, 1999) -
The roles of diet and SirT3 levels in mediating signaling network changes in insulin resistance
(Massachusetts Institute of Technology, 2013)The goal of my research is to understand the mechanism by which high fat diets mediate insulin sensitivity and the role SirT3 plays in high fat diet-induced insulin resistance. Insulin resistance is defined as the inability ... -
The spontaneous mutational spectrum of exon 2 and the high melting region of exon 3 of the human HPRT gene
(Massachusetts Institute of Technology, 1999) -
Streamlining and standardizing transcriptomic analysis in Amgen process development
(Massachusetts Institute of Technology, 2016)Building biological understanding of the Chinese Hamster Ovary (CHO) system used to manufacture therapeutic proteins is paramount to efficient CHO bioprocess optimization. This understanding can be built by analyzing and ... -
A study of inter-individual differences in the DNA damage response
(Massachusetts Institute of Technology, 2012)Agents that damage our DNA are omnipresent in our environment and inside our cells themselves. Left unrepaired, DNA damage can lead to premature aging, neurodegeneration and cancer. Humans have thus evolved intricate and ... -
Symmetric signaling by an asymmetric 1 erythropoietin : 2 erythropoietin receptor complex
(Massachusetts Institute of Technology, 2008)One erythropoietin molecule binds asymmetrically to two identical receptor monomers via erythropoietin site 1 and site 2, although it is unclear how asymmetry affects receptor activation and signaling. Here we report the ... -
Synthesis and characterization of oligonucleotides containing deoxyxanthosine : a probe for the mutagentic and genotoxic activity of an oxidized DNA base
(Massachusetts Institute of Technology, 1995) -
Synthesis and error correction methods in gene fabrication
(Massachusetts Institute of Technology, 2006)Gene Fabrication technology involves the development and optimization of methods relevant to the in vitro synthesis of any given target gene sequence(s) in the absence of template. The driving purpose of this field of ... -
Tau aggregation is heterogeneous across cases of sporadic Alzheimer's disease and is influenced by autophagy pathways in vitro
(Massachusetts Institute of Technology, 2020)Tau neurofibrillary tangles or aggregates are a common neuropathological feature found in a number of neurodegenerative conditions, including Alzheimer's disease. Understanding the kinetics of this aggregate build up, how ... -
Tissue-specific classification of alternatively spliced human exons
(Massachusetts Institute of Technology, 2007)Alternative splicing is involved in numerous cellular functions and is often disrupted and involved in disease. Previous research has identified methods to distinguish alternative conserved exons (ACEs) in human and mouse. ...
