Browsing Biological Engineering - Master's degree by Title
Now showing items 77-95 of 95
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The spontaneous mutational spectrum of exon 2 and the high melting region of exon 3 of the human HPRT gene
(Massachusetts Institute of Technology, 1999) -
Streamlining and standardizing transcriptomic analysis in Amgen process development
(Massachusetts Institute of Technology, 2016)Building biological understanding of the Chinese Hamster Ovary (CHO) system used to manufacture therapeutic proteins is paramount to efficient CHO bioprocess optimization. This understanding can be built by analyzing and ... -
A study of inter-individual differences in the DNA damage response
(Massachusetts Institute of Technology, 2012)Agents that damage our DNA are omnipresent in our environment and inside our cells themselves. Left unrepaired, DNA damage can lead to premature aging, neurodegeneration and cancer. Humans have thus evolved intricate and ... -
Symmetric signaling by an asymmetric 1 erythropoietin : 2 erythropoietin receptor complex
(Massachusetts Institute of Technology, 2008)One erythropoietin molecule binds asymmetrically to two identical receptor monomers via erythropoietin site 1 and site 2, although it is unclear how asymmetry affects receptor activation and signaling. Here we report the ... -
Synthesis and characterization of oligonucleotides containing deoxyxanthosine : a probe for the mutagentic and genotoxic activity of an oxidized DNA base
(Massachusetts Institute of Technology, 1995) -
Synthesis and error correction methods in gene fabrication
(Massachusetts Institute of Technology, 2006)Gene Fabrication technology involves the development and optimization of methods relevant to the in vitro synthesis of any given target gene sequence(s) in the absence of template. The driving purpose of this field of ... -
Tau aggregation is heterogeneous across cases of sporadic Alzheimer's disease and is influenced by autophagy pathways in vitro
(Massachusetts Institute of Technology, 2020)Tau neurofibrillary tangles or aggregates are a common neuropathological feature found in a number of neurodegenerative conditions, including Alzheimer's disease. Understanding the kinetics of this aggregate build up, how ... -
Tissue-specific classification of alternatively spliced human exons
(Massachusetts Institute of Technology, 2007)Alternative splicing is involved in numerous cellular functions and is often disrupted and involved in disease. Previous research has identified methods to distinguish alternative conserved exons (ACEs) in human and mouse. ... -
Tools to study the kinesin mechanome using optical tweezers
(Massachusetts Institute of Technology, 2009)Molecular motors play an important role in driving some of the most complex and important tasks in biological systems, ranging from transcribing RNA from a DNA template (Polymerases) to muscle contraction (Myosin) and ... -
Towards a carbon nanotube antibody sensor
(Massachusetts Institute of Technology, 2012)This work investigated single-walled carbon nanotube (SWNT)/polymer-protein A complexes for optically reporting antibody concentration via a change in near infrared fluorescent emission after antibody binding. SWNT have ... -
Towards a microfluidic disease detection deviced based on cellular adhesion differences
(Massachusetts Institute of Technology, 2006)There is a great need in the fields of biology, medicine, and pharmaceuticals to create high-throughput devices for the detection of specific cell states in a heterogeneous mixture of cells. The desire is to differentiate ... -
Transcriptional divergence and conservation of human and mouse erythropoiesis
(Massachusetts Institute of Technology, 2014)Mouse models have been used extensively for decades and have been instrumental in improving our understanding of mammalian erythropoiesis. Nonetheless, there are several examples of variation between human and mouse ... -
Transcriptional profiling of rat primary hepatocytes cultured in a 3D microfabricated liver reactor
(Massachusetts Institute of Technology, 2003) -
Transcriptional response of O⁶-methylguanine methyltransferase deficient yeast to methyl-N-nitro-N-nitrosoguanidine (MNNG)
(Massachusetts Institute of Technology, 2004)(cont.) of transcription factors and subsequently, induction of RNA processing (35% of genes incrementally induced) and kinases involved in protein phosphorylation. In the WT, the response was restricted to a transient ... -
Use of gene expression to characterize heterogeneous liver cell populations
(Massachusetts Institute of Technology, 2004)Non-parenchymal cells (NPC's) are integral to recreate the native hepatic microenvironment and necessary to maintain in vivo liver function. A variety of in vitro culture systems have been developed to address different ... -
Use Of synthetic solid scaffolds to mechanically support a chondrocyte-seeded peptide hydrogel for articular cartilage repair
(Massachusetts Institute of Technology, 2017)Post-traumatic osteoarthritis (PTOA) is a subtype of OA associated with cartilage defects caused by traumatic joint injury. Because articular cartilage has a limited innate healing response, due to its avascular, aneural, ... -
Using emergent self-organizing maps to identify marine group II archaea genomic fragments from uncharacterized microbial metagenomic sequences
(Massachusetts Institute of Technology, 2010)The validity and usefulness of clustering marine group II tetranucleotide signatures using emergent self-organizing maps was investigated. Fosmids from the HF200 library were chosen for sequencing based on end-sequence ... -
Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
(Massachusetts Institute of Technology, 2006)Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of ... -
Virulence factors in non-gastric Helicobacters
(Massachusetts Institute of Technology, 1998)
