Browsing Biological Engineering - Master's degree by Title
Now showing items 52-71 of 95
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Measuring mechanical properties of the tectorial membrane with a microfabricated probe
(Massachusetts Institute of Technology, 2004)Dynamic material properties of the isolated mouse tectorial membrane (TM) were quantified by applying sinusoidal shearing forces to the TM with a microfabricated probe (contact area 30 x 30 [micro]m²). Forces in the range ... -
A microfluidic platform for three-dimensional neuron culture
(Massachusetts Institute of Technology, 2007)Neurodegenerative diseases typically affect a limited number of specific neuronal subtypes, and the death of these neurons causes permanent loss of a specific motor function. Efforts to restore function would require ... -
Mitigating the effects of ribosome limitations on synthetic circuits via high-gain sRNA-mediated negative feedback
(Massachusetts Institute of Technology, 2015)Resource limitations in bacterial cells can present significant hurdles that preclude correct synthetic circuit behavior. In a simple circuit with one constitutively expressed protein and one protein whose expression is ... -
A modeling framework and toolset for simulation and characterization of the cochlea within the auditory system
(Massachusetts Institute of Technology, 2011)Purpose: This research develops a modeling approach and an implementation toolset to simulate reticular lamina displacement in response to excitation at the ear canal and to characterize the cochlear system in the frequency ... -
Modulation of the response to cisplatin by nitric oxide and reactive oxygen species in melanoma cells
(Massachusetts Institute of Technology, 2013)Malignant melanoma causes the highest mortality rate in skin cancers. Although cisplatin has proved efficacious in the treatment of various solid tumors, melanoma seems particularly resistant to this chemotherapeutic drug. ... -
Molecular mechanism of interactions between estrogen receptor and estrogen receptor selective genotoxins
(Massachusetts Institute of Technology, 2000)Although one million new breast cancer cases arise each year worldwide, therapies to treat the disease are limited. Conventional treatments including the chemotherapeutic agent, Tamoxifen, have had only limited success, ... -
Monogenic, multigenic, and polygenic determinants of cancer risk
(Massachusetts Institute of Technology, 2002)A formal series of conditions of lifetime genetic risk of cancer is explored, and algebra is provided for applications in human population genetics. Risks are considered in terms of alleles necessary and/or sufficient for ... -
Multi-scale analysis of cardiac myoarchitecture
(Massachusetts Institute of Technology, 2008)The distribution and generation of force within the myocardium during normal contractility is dictated by the tissue's underlying 3D myoarchitecture. The presence of disordered myoarchitecture may in turn constitute the ... -
Murine embryonic stem cells and hypoxia : growth kinetics, metabolism, and plating efficiency
(Massachusetts Institute of Technology, 2004)There is reason to believe that embryonic stem cells would grow favorably in a hypoxic environment. These cells come from the pre-implantation embryo, whose native environment is the hypoxic mammalian reproductive tract. ... -
A new mouse model to probe the role of aflatoxin B₁ in liver carcinogenesis
(Massachusetts Institute of Technology, 2010)One and a half million new cancer cases are reported each year in the United States. Despite this overwhelming burden of disease, current preventative treatments and early detection techniques are inadequate. With cancers, ... -
A novel bioengineering platform using functionalized self-assembly peptides to enhance CYP3A2 activity in modified rat hepatocyte sandwich cultures
(Massachusetts Institute of Technology, 2007)Isolated hepatocytes removed from their microenvironment soon lose their hepatospecific functions when cultured. Highly oxygen-demanding hepatocytes are commonly maintained under oxygen-deficient culture conditions, limited ... -
A novel high-throughput in-cell Western assay for the quantitative measurement of signaling dynamics in DNA damage signaling networks : cell decision processes in response to DNA double strand breaks
(Massachusetts Institute of Technology, 2006)Following exposure to DNA damage, cells initiate a stress response involving multiple protein kinase signaling cascades. The DNA damage response results in one of several possible cell-fate decisions, or cellular responses: ... -
A novel nanodelivery system for combination tumor therapy
(Massachusetts Institute of Technology, 2004)Anti-angiogenic therapy offers many benefits over traditional cytotoxic chemotherapy including fewer toxic side effects and the reduced development of drug resistance. Anti-angiogenics alone have not proven effective in ... -
Optimization of cell adhesion environments for a liver cell bioreactor
(Massachusetts Institute of Technology, 2006)The MilliF bioreactor offers great potential for the formation of i vivo-like liver tissue outside the body, making it a valuable tool for applications such as drug toxicity models and biosensors. Cell adhesion is an ... -
Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
(Massachusetts Institute of Technology, 2006)Nonviral vector research and development has been stunted by a lack of knowledge and understanding of how vectors are trafficked within the cell. Research currently involves mass screenings of different combinations of ... -
Process Improvement in Biotech : Dealing with High Complexity Processes
(Massachusetts Institute of Technology, 2009)Across numerous conventional manufacturing sites, process improvement initiatives have been shown to increase production capabilities while decreasing costs - all without a required system- wide overhaul of the manufacturing ... -
Quantitation of 8-oxoG and strand breaks in pUC19 plasmid DNA after exposure to oxygen radical generating systems
(Massachusetts Institute of Technology, 1995) -
Quantitative analysis of TLR-4-mediated cell responses in murine macrophages
(Massachusetts Institute of Technology, 2008)TLR-4 is essential in host defense against bacterial infection. By recognition of specific pathogen-associated molecular patterns such as lipopolysaccharide (LPS), TLR-4 can in tandem initiate a pair of downstream signaling ... -
A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage
(Massachusetts Institute of Technology, 2011)Objectives: 1) To perform a quantitative comparison of proteins released to media on combination with cytokine (IL-1[beta[ or TNF-[alpha]) and Injury as compared to either treatment alone, and to thus identify proteins ... -
A rapid, flexible and scalable DNA assembly platform for genome engineering and regulated gene expression applications in Plasmodium falciparum
(Massachusetts Institute of Technology, 2015)Plasmodium falciparum is the deadliest malaria parasite. There is no approved vaccine to prevent this disease, and resistance to available antimalarial drugs is becoming widespread. Identification of parasite genes essential ...
