Browsing Biological Engineering - Ph.D. / Sc.D. by Title
Now showing items 220-239 of 347
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Nanopores, megatonnes, and milliseconds : exploring engineered peptides as antimicrobial, carbon-capture,and biocatalytic agents
(Massachusetts Institute of Technology, 2012)This work investigates the roles that peptides play in the fields of antimicrobials, surface functionalization, carbon capture, and biocatalysis. The results demonstrate that peptides, sometimes dismissed for their lack ... -
Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
(Massachusetts Institute of Technology, 2005)Micro- and nanofabrication approaches have dramatically changed our society through their use in microelectronics and telecommunication industries. These engineering tools are also useful for many biological applications ... -
Nanoscale biomolecular mapping in cells and tissues with expansion microscopy
(Massachusetts Institute of Technology, 2019)The ability to map the molecular organization of cells and tissues with nanoscale precision would open the door to understanding their biological functions as well as the mechanisms that lead to pathologies. Though recent ... -
Nanostructures templated on biological scaffolds for light harvesting, energy transfer, charge transfer, and redox reactions
(Massachusetts Institute of Technology, 2010)Solar energy provides an unparalleled promise to generate enormous amounts of clean energy. As the solar industry grows rapidly with a focus on power generation, new, but equally important challenges are emerging, including ... -
Network analyses for functional genomic screens in cancer
(Massachusetts Institute of Technology, 2016)Gene interference screens are a widely adopted and popular tool for uncovering gene function but imperfections in the technology limit the power of these investigations. There are many completed and on-going RNAi investigations ... -
New protein engineering approaches for potentiating and studying antibody-based EGFR antagonism
(Massachusetts Institute of Technology, 2019)A variety of cancers are marked by the over-expression and over-activity of the EGF receptor (EGFR), rendering this protein an attractive therapeutic target. Anti-EGFR therapeutics are a mainstay of clinical practice for ... -
Next-generation sequencing as a tool for investigating the in vivo biological consequences of DNA lesions and its applications on the ethenoguanine, 8-oxoguanine and 1,3-butadiene-induced lesions
(Massachusetts Institute of Technology, 2017)DNA damaging agents produce a plethora of DNA lesions, which can lead to various outcomes. In order to understand the biological consequences of DNA lesions, and hence gain insights into the carcinogenic mechanisms of DNA ... -
Nitric oxide : cellular effects in vitro and in vivo
(Massachusetts Institute of Technology, 2001)The overall aim of this project was to investigate various cellular responses and toxic effects of nitric oxide, NO' and in vitro and in vivo. Nitric oxide gives rise to a complex spectrum of reactive species in oxygenated ... -
Noncovalent adsorption of nucleotides in gold nanoparticle DNA conjugates : bioavailability at the bio-nano interface
(Massachusetts Institute of Technology, 2008)The practical viability of biomolecule-nanostructure hybrids depends critically on the functional and structural stability of biomolecules in application environments. Noncovalent interactions of biochemical functional ... -
Novel applications and methods for the computer-aided understanding and design of enzyme activity
(Massachusetts Institute of Technology, 2018)Despite great progress over the past several decades in the development and application of computer-aided tools for engineering enzymes for a vast array of industrial applications. rational enzyme design remains an ongoing ... -
A novel DNA damage quantification platform enables high throughput screening for genes that impact DNA double strand breaks
(Massachusetts Institute of Technology, 2018)DNA is the blueprint of life, and the high fidelity transmission of genetic information from parent cells to progeny is essential for an organism's viability. However, our genomes are constantly being damaged by reactive ... -
Novel tools for sequence and epitope analysis of glycosaminoglycans
(Massachusetts Institute of Technology, 2007)Our understanding of glycosaminoglycan (GAG) biology has been limited by a lack of sensitive and efficient analytical tools designed to deal with these complex molecules. GAGs are heterogeneous and often sulfated linear ... -
Nucleic acid modifications in bacterial pathogens - impact on pathogenesis, diagnosis, and therapy
(Massachusetts Institute of Technology, 2014)Nucleic acids are subject to extensive chemical modification by all organisms. These modifications display incredible structural diversity, and some are essential for survival. Intriguingly, several of these modifications ... -
Nucleobase deamination as a biomarker of inflammatory processes
(Massachusetts Institute of Technology, 2005)(cont.) kinetic studies. Moderate increases ([approximately]30%) in nucleobase deamination products were observed in the SJL mice bearing the RcsX tumor, but the biological meaning of these increases awaits studies of DNA ... -
N⁶-formylation of lysine : a pathological secondary modification of proteins
(Massachusetts Institute of Technology, 2013)There is increasing recognition that aberrant protein modifications play an important role in the pathophysiology of inflammation and oxidative stress in cells. We recently discovered that N⁶-formylation of lysine is an ... -
Olfactory-related receptors : methods towards enabling structural and functional studies
(Massachusetts Institute of Technology, 2011)Mammalian noses can detect and distinguish an inestimable number of odors at minute concentrations. Four classes of G protein-coupled receptors (GPCRs) are responsible for this remarkable sensitivity: olfactory receptors ... -
Optimization of primary endothelial culture methods and assessment of cell signaling pathways in the context of inflammation
(Massachusetts Institute of Technology, 2012)Tissue engineering is a potentially valuable tool for clinical treatment of diseases where host tissues or organs need to be replaced. Progression of engineering metabolically complex organs and tissues has been severely ... -
Optimizing chondrogenic factors and protein delivery methods for cartilage repair
(Massachusetts Institute of Technology, 2012)Joint injuries are common and often result in damage to cartilage, which has a limited ability to repair itself. Tissue engineering is a promising approach for improving cartilage healing in which biomaterials and chemical ... -
Overcoming dendritic cell-mediated suppression of T cell responses in a prostate tumor environment
(Massachusetts Institute of Technology, 2010)Prostate cancer is the most prevalent malignancy in American men, leading to significant mortality each year. This is in part due to a lack of effective treatments for advanced disease. The prostate is considered an ideal ... -
Oxidative DNA damage : mutagenic properties of 5-hydorxycytosine, 5-hydroxyuracil and uracil glycol in Eschericia coli
(Massachusetts Institute of Technology, 1998)
