Browsing Biological Engineering - Ph.D. / Sc.D. by Title
Now showing items 1-20 of 309
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A 3D synthetic extracellular matrix that can be dissolved to interrogate the cellular local microenvironment on demand
(Massachusetts Institute of Technology, 2016)In vitro multi-cellular 3D cultures can provide tremendous insight into pathologies arising from dysregulation of extracellular communication by recapitulating key features of the microenvironment, such as ECM-cytokine ... -
Alkyladenine DNA glycosylase (Aag)-dependent cell-specific responses to alkylating agents
(Massachusetts Institute of Technology, 2016)Methylating agents are ubiquitous in our internal and external environments and can cause damage to all cellular components, including our DNA. If left unrepaired, methylated DNA can cause mutations, cell death, and disease, ... -
Alkylation damage in mammalian cells : an analysis of the mutagenic potential of O4-methylthymine adducts
(Massachusetts Institute of Technology, 1994) -
Amphiphilic gold nanoparticles: mechanisms for interaction with membranes and applications in drug and vaccine delivery
(Massachusetts Institute of Technology, 2014)Materials that can interact with and transit membranes without toxic bilayer disruption or poration are of great interest in the drug delivery field. These materials can presumably bypass endocytosis to directly enter the ... -
Analysis of biological and chemical systems using information theoretic approximations
(Massachusetts Institute of Technology, 2010)The identification and quantification of high-dimensional relationships is a major challenge in the analysis of both biological and chemical systems. To address this challenge, a variety of experimental and computational ... -
Analysis of community cancer mortality rates
(Massachusetts Institute of Technology, 2001)Residents of small communities or neighborhoods often observe what appears to them to be an excessively high number of cancer cases. Some of these observations are brought to the attention of public health officials in the ... -
Analysis of sequence-selective guanine oxidation by biological agents
(Massachusetts Institute of Technology, 2008)Oxidatively damaged DNA has been strongly associated with cancer, chronic degenerative diseases and aging. Guanine is the most frequently oxidized base in the DNA, and generation of a guanine radical cation (G'") as an ... -
Analysis of the structural changes caused by positive DNA supercoiling
(Massachusetts Institute of Technology, 2007)The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ... -
The androgen receptor independent mechanism of toxicity of the novel anti-tumor agent 11[beta]-dichloro
(Massachusetts Institute of Technology, 2009)Inspired by the toxicity mechanism of cisplatin in testicular cancer, a series of bi-functional genotoxicants has been designed that supplement their DNA damaging properties with the ability to interact with tumor specific ... -
An apparatus for high throughput muscle cell experimentation
(Massachusetts Institute of Technology, 2006)The cardiac ventricular muscle cell (myocyte) is a key experimental system for exploring the mechanical properties of the diseased and healthy heart. The myocyte experimental model provides a higher level of physiological ... -
Application of endostatin using nonviral gene delivery toward the regeneration of articular cartilage
(Massachusetts Institute of Technology, 2011)Articular cartilage is avascular, and defects have limited capacity for spontaneous healing. Angiogenesis may interfere with maturation of naturally avascular tissues. Our rationale is that the use of endostatin, a potent ... -
Applications of genome editing for disease modeling in mice
(Massachusetts Institute of Technology, 2016)The genome holds the blueprint of life and heredity. In the case of the mammalian genome it is comprised of billions of DNA bases grouped into elements that we are beginning to understand (ie genes) and others we know ... -
Applying engineering principles to the design and construction of transcriptional devices
(Massachusetts Institute of Technology, 2008)The aim of this thesis is to consider how fundamental engineering principles might best be applied to the design and construction of engineered biological systems. I begin by applying these principles to a key application ... -
Arming adoptively transferred T-cells with drug-loaded nanoparticles for cancer immunotherapy
(Massachusetts Institute of Technology, 2016)In adoptive cell therapy (ACT), autologous tumor-specific T-cells isolated from cancer patients or genetically engineered lymphocytes are activated and expanded ex vivo, then infused back into the individual to eliminate ... -
Bacterial genes and genome dynamics in the environment
(Massachusetts Institute of Technology, 2013)One of the most marvelous features of microbial life is its ability to thrive in such diverse and dynamic environments. My scientific interest lies in the variety of modes by which microbial life accomplishes this feat. ... -
Bayesian network models of biological signaling pathways
(Massachusetts Institute of Technology, 2006)Cells communicate with other cells, and process cues from their environment, via signaling pathways, in which extracellular cues trigger a cascade of information flow, causing signaling molecules to become chemically, ... -
Bioimage informatics for understanding the effects of chemotherapy on cellular signaling, structure, and function
(Massachusetts Institute of Technology, 2017)Chemotherapy is widely used in the treatment of solid tumors, but its effects are often associated with cancer relapse, metastasis, and drug resistance. The biological mechanisms that drive the structural and functional ... -
Biomolecular and computational frameworks for genetic circuit design
(Massachusetts Institute of Technology, 2017)Living cells naturally use gene regulatory networks termed "genetic circuits" to exhibit complex behaviors such as signal processing, decision-making, and spatial organization. The ability to rationally engineer genetic ... -
Biophysical characterization of high affinity engineered single chain Fv antibody fragments
(Massachusetts Institute of Technology, 2004)High affinity antibody binding interactions are important for both pharmaceutical and biotechnological uses. However, designing higher affinity interactions has remained difficult. Both high affinity interactions from ... -
Biophysical responses of lymphocytes to environmental stress
(Massachusetts Institute of Technology, 2016)Cellular biophysical properties both reflect and influence cell state. These parameters represent the consequences of the interactions of multiple molecular events, and thus may reveal information otherwise obscured when ...
