Regeneration of Alveolar Type I and II Cells from Scgb1a1-Expressing Cells following Severe Pulmonary Damage Induced by Bleomycin and Influenza
Author(s)
Zheng, Dahai; Limmon, Gino V.; Yin, Lu; Leung, Nicola H. N.; Yu, Hanry; Chow, Vincent T. K.; Chen, Jianzhu; ... Show more Show less
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The lung comprises an extensive surface of epithelia constantly exposed to environmental insults. Maintaining the integrity of the alveolar epithelia is critical for lung function and gaseous exchange. However, following severe pulmonary damage, what progenitor cells give rise to alveolar type I and II cells during the regeneration of alveolar epithelia has not been fully determined. In this study, we have investigated this issue by using transgenic mice in which Scgb1a1-expressing cells and their progeny can be genetically labeled with EGFP. We show that following severe alveolar damage induced either by bleomycin or by infection with influenza virus, the majority of the newly generated alveolar type II cells in the damaged parenchyma were labeled with EGFP. A large proportion of EGFP-expressing type I cells were also observed among the type II cells. These findings strongly suggest that Scgb1a1-expressing cells, most likely Clara cells, are a major cell type that gives rise to alveolar type I and II cells during the regeneration of alveolar epithelia in response to severe pulmonary damage in mice..
Date issued
2012-10Department
Massachusetts Institute of Technology. Department of Biology; Singapore-MIT Alliance in Research and Technology (SMART); Koch Institute for Integrative Cancer Research at MITJournal
PLoS One
Publisher
Public Library of Science
Citation
Zheng, Dahai et al. “Regeneration of Alveolar Type I and II Cells from Scgb1a1-Expressing Cells Following Severe Pulmonary Damage Induced by Bleomycin and Influenza.” Ed. Saverio Bellusci. PLoS ONE 7.10 (2012): e48451. Web.
Version: Final published version
ISSN
1932-6203