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dc.contributor.authorZhao, Weian
dc.contributor.authorSchafer, Sebastian
dc.contributor.authorChoi, Jonghoon
dc.contributor.authorYamanaka, Yvonne Joy
dc.contributor.authorLombardi, Maria L.
dc.contributor.authorBose, Suman
dc.contributor.authorCarlson, Alicia L.
dc.contributor.authorPhillips, Joseph A.
dc.contributor.authorTeo, Weisuong
dc.contributor.authorDroujinine, Ilia A.
dc.contributor.authorCui, Cheryl
dc.contributor.authorJain, Rakesh K.
dc.contributor.authorLammerding, Jan
dc.contributor.authorLove, J. Christopher
dc.contributor.authorLin, Charles
dc.contributor.authorSarkar, Debanjan
dc.contributor.authorKarnik, Rohit
dc.contributor.authorKarp, Jeffrey Michael
dc.date.accessioned2013-05-13T20:54:09Z
dc.date.available2013-05-13T20:54:09Z
dc.date.issued2011-07
dc.date.submitted2010-12
dc.identifier.issn1748-3387
dc.identifier.issn1748-3395
dc.identifier.urihttp://hdl.handle.net/1721.1/78868
dc.descriptionAuthor Manuscript 2012 August 1.en_US
dc.description.abstractThe ability to explore cell signalling and cell-to-cell communication is essential for understanding cell biology and developing effective therapeutics. However, it is not yet possible to monitor the interaction of cells with their environments in real time. Here, we show that a fluorescent sensor attached to a cell membrane can detect signalling molecules in the cellular environment. The sensor is an aptamer (a short length of single-stranded DNA) that binds to platelet-derived growth factor (PDGF) and contains a pair of fluorescent dyes. When bound to PDGF, the aptamer changes conformation and the dyes come closer to each other, producing a signal. The sensor, which is covalently attached to the membranes of mesenchymal stem cells, can quantitatively detect with high spatial and temporal resolution PDGF that is added in cell culture medium or secreted by neighbouring cells. The engineered stem cells retain their ability to find their way to the bone marrow and can be monitored in vivo at the single-cell level using intravital microscopy.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant HL097172)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant HL095722)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant DE019191)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant NIAID 5RC1AI086152)en_US
dc.description.sponsorshipCharles A. Dana Foundationen_US
dc.description.sponsorshipAmerican Heart Association (Grant 0970178N)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Graduate Fellowship)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nnano.2011.101en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePubMed Centralen_US
dc.titleCell-surface sensors for real-time probing of cellular environmentsen_US
dc.typeArticleen_US
dc.identifier.citationZhao, Weian, Sebastian Schafer, Jonghoon Choi, et al. 2011Cell-surface Sensors for Real-time Probing of Cellular Environments. Nature Nanotechnology 6(8): 524–531.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorZhao, Weian
dc.contributor.mitauthorSchafer, Sebastian
dc.contributor.mitauthorChoi, Jonghoon
dc.contributor.mitauthorYamanaka, Yvonne Joy
dc.contributor.mitauthorBose, Suman
dc.contributor.mitauthorPhillips, Joseph A.
dc.contributor.mitauthorTeo, Weisuong
dc.contributor.mitauthorDroujinine, Ilia A.
dc.contributor.mitauthorCui, Cheryl
dc.contributor.mitauthorLove, J. Christopher
dc.contributor.mitauthorSarkar, Debanjan
dc.contributor.mitauthorKarnik, Rohit
dc.contributor.mitauthorKarp, Jeffrey Michael
dc.relation.journalNature Nanotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsZhao, Weian; Schafer, Sebastian; Choi, Jonghoon; Yamanaka, Yvonne J.; Lombardi, Maria L.; Bose, Suman; Carlson, Alicia L.; Phillips, Joseph A.; Teo, Weisuong; Droujinine, Ilia A.; Cui, Cheryl H.; Jain, Rakesh K.; Lammerding, Jan; Love, J. Christopher; Lin, Charles P.; Sarkar, Debanjan; Karnik, Rohit; Karp, Jeffrey M.en
dc.identifier.orcidhttps://orcid.org/0000-0003-0588-9286
dc.identifier.orcidhttps://orcid.org/0000-0002-5921-3436
dc.identifier.orcidhttps://orcid.org/0000-0002-0160-8742
dc.identifier.orcidhttps://orcid.org/0000-0003-0921-3144
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US


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