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dc.contributor.authorChapin, Stephen C.
dc.contributor.authorDoyle, Patrick S.
dc.date.accessioned2013-05-30T18:04:54Z
dc.date.available2013-05-30T18:04:54Z
dc.date.issued2011-08
dc.date.submitted2011-06
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.urihttp://hdl.handle.net/1721.1/79043
dc.description.abstractThere is great demand for flexible biomolecule analysis platforms that can precisely quantify very low levels of multiple targets directly in complex biological samples. Herein we demonstrate multiplexed quantification of microRNAs (miRNAs) on encoded hydrogel microparticles with subfemtomolar sensitivity and single-molecule reporting resolution. Rolling circle amplification (RCA) of a universal adapter sequence that is ligated to all miRNA targets captured on gel-embedded probes provides the ability to label each target with multiple fluorescent reporters and eliminates the possibility of amplification bias. The high degree of sensitivity achieved by the RCA scheme and the resistance to fouling afforded by the use of gel particles are leveraged to directly detect miRNA in small quantities of unprocessed human serum samples without the need for RNA extraction or target-amplification steps. This versatility has powerful implications for the development of rapid, noninvasive diagnostic assays.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH Grant R21EB008814)en_US
dc.description.sponsorshipRagon Institute of MGH, MIT and Harvarden_US
dc.language.isoen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/ac201618ken_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleUltrasensitive Multiplexed MicroRNA Quantification on Encoded Gel Microparticles Using Rolling Circle Amplificationen_US
dc.typeArticleen_US
dc.identifier.citationChapin, Stephen C., and Patrick S. Doyle. Ultrasensitive Multiplexed MicroRNA Quantification on Encoded Gel Microparticles Using Rolling Circle Amplification. Analytical Chemistry 83, no. 18 (September 15, 2011): 7179-7185.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorChapin, Stephen C.en_US
dc.contributor.mitauthorDoyle, Patrick S.en_US
dc.relation.journalAnalytical Chemistryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChapin, Stephen C.; Doyle, Patrick S.en_US
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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