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dc.contributor.authorXu, Xiaofeng
dc.contributor.authorEfremov, Artem K.
dc.contributor.authorLi, Ang
dc.contributor.authorLai, Lipeng
dc.contributor.authorDao, Ming
dc.contributor.authorCao, Jianshu
dc.contributor.authorLim, Chwee-Teck
dc.date.accessioned2013-07-02T18:22:40Z
dc.date.available2013-07-02T18:22:40Z
dc.date.issued2013-05
dc.date.submitted2013-01
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/79409
dc.description.abstractMalaria is one of the most widespread and deadly human parasitic diseases caused by the Plasmodium (P.) species with the P.falciparum being the most deadly. The parasites are capable of invading red blood cells (RBCs) during infection. At the late stage of parasites’ development, the parasites export proteins to the infected RBCs (iRBC) membrane and bind to receptors of surface proteins on the endothelial cells that line microvasculature walls. Resulting adhesion of iRBCs to microvasculature is one of the main sources of most complications during malaria infection. Therefore, it is important to develop a versatile and simple experimental method to quantitatively investigate iRBCs cytoadhesion and binding kinetics. Here, we developed an advanced flow based adhesion assay to demonstrate that iRBC’s adhesion to endothelial CD36 receptor protein coated channels is a bistable process possessing a hysteresis loop. This finding confirms a recently developed model of cell adhesion which we used to fit our experimental data. We measured the contact area of iRBC under shear flow at different stages of infection using Total Internal Reflection Fluorescence (TIRF), and also adhesion receptor and ligand binding kinetics using Atomic Force Microscopy (AFM). With these parameters, we reproduced in our model the experimentally observed changes in adhesion properties of iRBCs accompanying parasite maturation and investigated the main mechanisms responsible for these changes, which are the contact area during the shear flow as well as the rupture area size.en_US
dc.description.sponsorshipGlobal Enterprise for Micro-Mechanics and Molecular Medicineen_US
dc.description.sponsorshipUnited States. Dept. of Defense (DOD-ARO (W 911 NF-09-0480))en_US
dc.description.sponsorshipSingapore–MIT Alliance for Research and Technology ((SMART) Fellowship)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (NSF Grant No.1112825)en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0064763en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titleProbing the Cytoadherence of Malaria Infected Red Blood Cells under Flowen_US
dc.typeArticleen_US
dc.identifier.citationXu, Xiaofeng, Artem K. Efremov, Ang Li, Lipeng Lai, Ming Dao, Chwee Teck Lim, and Jianshu Cao. Probing the Cytoadherence of Malaria Infected Red Blood Cells Under Flow. Edited by Georges Snounou. PLoS ONE 8, no. 5 (May 28, 2013): e64763.en_US
dc.contributor.departmentMIT-SUTD Collaboration Officeen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.contributor.departmentSingapore-MIT Alliance in Research and Technology (SMART)en_US
dc.contributor.mitauthorLim, Chwee Tecken_US
dc.contributor.mitauthorDao, Mingen_US
dc.contributor.mitauthorEfremov, Artem K.en_US
dc.contributor.mitauthorXu, Xiaofengen_US
dc.contributor.mitauthorLai, Lipengen_US
dc.contributor.mitauthorLi, Angen_US
dc.contributor.mitauthorCao, Jianshuen_US
dc.relation.journalPLoS ONEen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsXu, Xiaofeng; Efremov, Artem K.; Li, Ang; Lai, Lipeng; Dao, Ming; Lim, Chwee Teck; Cao, Jianshuen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7616-7809
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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