HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy
Author(s)JnBaptiste, Courtney Kenneil; Michaud, Joelle; Praz, Viviane; James Faresse, Nicole; Tyagi, Shweta; Schutz, Frederic; Herr, Winship; ... Show more Show less
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In human transcriptional regulation, DNA-sequence-specific factors can associate with intermediaries that orchestrate interactions with a diverse set of chromatin-modifying enzymes. One such intermediary is HCFC1 (also known as HCF-1). HCFC1, first identified in herpes simplex virus transcription, has a poorly defined role in cellular transcriptional regulation. We show here that, in HeLa cells, HCFC1 is observed bound to 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCFC1-binding sites revealed three sequence motifs associated with the binding of (1) ZNF143 and THAP11 (also known as Ronin), (2) GABP, and (3) YY1 sequence-specific transcription factors. Subsequent analysis revealed colocalization of HCFC1 with these four transcription factors at ∼90% of the 5400 HCFC1-bound promoters. These studies suggest that a relatively small number of transcription factors play a major role in HeLa-cell transcriptional regulation in association with HCFC1.
DepartmentMassachusetts Institute of Technology. Department of Biology
Cold Spring Harbor Laboratory Press
Michaud, J., V. Praz, N. James Faresse, C. K. JnBaptiste, S. Tyagi, F. Schutz, and W. Herr. “HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy.” Genome Research 23, no. 6 (June 1, 2013): 907-916. © 2013, Published by Cold Spring Harbor Laboratory Press
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