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FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions

Author(s)
Imperiali, Barbara; Socher, Elke Ricarda
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Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/
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Abstract
The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals.
Date issued
2012-12
URI
http://hdl.handle.net/1721.1/84588
Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Journal
ChemBioChem
Publisher
Wiley Blackwell
Citation
Socher, Elke, and Barbara Imperiali. “FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions.” ChemBioChem 14, no. 1 (January 2, 2013): 53-57.
Version: Author's final manuscript
ISSN
14394227
1439-7633

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