Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions

Author(s)
Sainlos, Matthieu; Iskenderian, Wendy S.; Olivier, Nelson B.; Choquet, Daniel; Imperiali, Barbara
Thumbnail
DownloadImperiali_Caged mono paper and SI.pdf (3.402Mb)
OPEN_ACCESS_POLICY
Terms of use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Metadata
Show full item record
Abstract
We report a general method for light-assisted control of interactions of PDZ domain binding motifs with their cognate domains by the incorporation of a photolabile caging group onto the essential C-terminal carboxylate binding determinant of the motif. The strategy was implemented and validated for both simple monovalent and biomimetic divalent ligands, which have recently been established as powerful tools for acute perturbation of native PDZ domain-dependent interactions in live cells.
Date issued
2013-03
URI
http://hdl.handle.net/1721.1/86182
Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Sainlos, Mathieu, Wendy S. Iskenderian-Epps, Nelson B. Olivier, Daniel Choquet, and Barbara Imperiali. "Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions." Journal of the American Chemical Society, 2013, 135 (12), pp 4580–4583.
Version: Author's final manuscript
ISSN
0002-7863
1520-5126
Collections