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Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions

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Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions

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Author: Sainlos, Matthieu; Iskenderian-Epps, Wendy S.; Olivier, Nelson B.; Choquet, Daniel; Imperiali, Barbara
Department: Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Publisher: American Chemical Society
Date Issued: 2013-03
Abstract:
We report a general method for light-assisted control of interactions of PDZ domain binding motifs with their cognate domains by the incorporation of a photolabile caging group onto the essential C-terminal carboxylate binding determinant of the motif. The strategy was implemented and validated for both simple monovalent and biomimetic divalent ligands, which have recently been established as powerful tools for acute perturbation of native PDZ domain-dependent interactions in live cells.
URI: http://hdl.handle.net/1721.1/86182
ISSN: 0002-7863
1520-5126
Citation: Sainlos, Mathieu, Wendy S. Iskenderian-Epps, Nelson B. Olivier, Daniel Choquet, and Barbara Imperiali. "Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions." Journal of the American Chemical Society, 2013, 135 (12), pp 4580–4583.
Version: Author's final manuscript
Terms of Use: Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Published As: http://dx.doi.org/10.1021/ja309870q
Journal: Journal of the American Chemical Society

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