An adsorption chromatography assay to probe bulk particle transport through hydrogels
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Ribbeck_An adsorption.pdf
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Author(s)
Vladescu, I.
Lieleg, Oliver
Jang, S.
Ribbeck, Katharina
Date Issued
September 2011
Journal
Journal of Pharmaceutical Sciences
Publisher
Wiley Blackwell
Citation
Vladescu, I., O. Lieleg, S. Jang, and Katharina Ribbeck. “An Adsorption Chromatography Assay to Probe Bulk Particle Transport through Hydrogels.” J. Pharm. Sci. 101, no. 1 (September 8, 2011): 436–442.
Version
Author's final manuscript
Abstract
Biopolymer-based hydrogels such as mucus and the basal lamina play a key role in biology, where they control the exchange of material between different compartments. They also pose a barrier that needs to be overcome for successful drug delivery. Characterizing the permeability properties of such hydrogels is mandatory for the development of suitable drug delivery vectors and pharmaceutics. Here, we present an experimental method to measure bulk particle transport through hydrogels. We validate our assay by applying it to mucin hydrogels and show that the permeability properties of these mucin hydrogels can be modulated by polymer density and pH, in agreement with previous results obtained from single particle tracking. The method we present here is easy to handle, inexpensive, and high-throughput compatible. It is also a suitable platform for the design and screening of drugs that aim at modifying the barrier properties of hydrogels. This system can also aid in the characterization and development of synthetic gels for a range of biomedical applications.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1002/jps.22737
