The evolution of mRNA splicing in mammals
Author(s)Merkin, Jason Jay
Evolution of mRNA splicing in mammalian tissues
Evolution of messenger ribonucleic acid splicing in mammalian tissues
Massachusetts Institute of Technology. Department of Biology.
Christopher B. Burge.
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In this thesis, I describe investigations into the evolution of splicing in mammals. I first investigate a small class of alternative splicing events, tandem splice sites, and show how they are used to introduce and remove coding sequence in a species-specific manner. I then describe the generation and analysis of a large RNA-seq dataset from 9 matched tissues in 5 species, with the aim to investigate the evolution of splicing in mammals. I first investigate the evolution of exons that predate the most ancient divergence of species studied, finding that their splicing is frequently poorly conserved. For a subset of these exons, I identify unique regulatory properties and provide evidence linking alternative splicing to phosphorylation potential of proteins. I then consider sources of novel exons, in these species. I use these and other published data to identify one way in which splicing of novel exons impacts the biology of the cell. I also present evidence implicating genomic indels in exon creation and splicing variation.
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2014.Title as it appears in MIT commencement exercises program, June 6, 2014: The evolution of mRNA splicing in mammalian tissues Cataloged from PDF version of thesis.Includes bibliographical references (pages 167-172).
DepartmentMassachusetts Institute of Technology. Department of Biology.; Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology