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dc.contributor.advisorRon Weiss.en_US
dc.contributor.authorSun, Jingjing, Ph. D. Massachusetts Institute of Technologyen_US
dc.contributor.otherMassachusetts Institute of Technology. Department of Biological Engineering.en_US
dc.date.accessioned2014-10-08T15:22:13Z
dc.date.available2014-10-08T15:22:13Z
dc.date.copyright2014en_US
dc.date.issued2014en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/90678
dc.descriptionThesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2014.en_US
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references (pages 155-165).en_US
dc.description.abstractCommunities of microorganisms are found nearly ubiquitously on earth. They survive and proliferate through interactions within and between microbial species, which are mediated by the exchange of small signaling modules. Understanding how they regulate the interactions is both crucial and challenging, with applications including industrial biotechnology, human health and environmental sustainability. In microbial ecology, researchers have been trying to culture pure and mixed species in different conditions to elucidate the rules behind the interactions. However, the studies have been complicated by multiple variables at both the genotype and phenotype levels. To address these challenges, I demonstrate a synthetic ecological system as a proof of principle to observe microbial population level behaviors. Using a formalized design process and engineering principles, I design and construct a synthetic multi-module ecological system for population homeostasis. The synthetic ecological system consists of four functionally distinct modules - quorum sensing, high threshold killing, low threshold killing, and intermediate rescuing modules. The system is able to maintain the yeast population within a programmable range in liquid culture. However, when the same system is studied in solid medium, heterogeneity in growth rate and population size is observed. To further study the heterogeneity issue in solid medium, I develop a cell deposition platform to evaluate sub-population level or even single-cell level behavior. With a commercial Nano eNabler machine, cells with pre-defined patterns are deposited on agarose surface. This technique can be used to study microbial communities in a spatially distributed fashion.en_US
dc.description.statementofresponsibilityby Jingjing Sun.en_US
dc.format.extent165 pagesen_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsMIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectBiological Engineering.en_US
dc.titleTowards synthetic ecology : genetically programmable 4-module population control system in yeasten_US
dc.typeThesisen_US
dc.description.degreePh. D.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineering
dc.identifier.oclc890466343en_US


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