Show simple item record

dc.contributor.authorRoh, Young Hoon
dc.contributor.authorDeng, Zhou J.
dc.contributor.authorShopsowitz, Kevin
dc.contributor.authorMorton, Stephen Winford
dc.contributor.authorHammond, Paula T
dc.date.accessioned2014-11-17T17:57:31Z
dc.date.available2014-11-17T17:57:31Z
dc.date.issued2013-12
dc.date.submitted2013-10
dc.identifier.issn16136810
dc.identifier.issn1613-6829
dc.identifier.urihttp://hdl.handle.net/1721.1/91594
dc.description.abstractInorganic nanostructures have been used extensively to package nucleic acids into forms useful for therapeutic applications. Here we report that the two products of transcription, RNA and inorganic pyrophosphate, can self-assemble to form composite microsponge structures composed of nanocrystalline magnesium pyrophosphate sheets (Mg[subscript 2]P[subscript 2]O[subscript 7]•3.5H[subscript 2]O) with RNA adsorbed to their surfaces. The microsponge particles contain high loadings of RNA (15–21 wt.%) that are protected from degradation and can be obtained through a rolling circle mechanism as large concatemers capable of mediating RNAi. The morphology of the RNAi microsponges is influenced by the time-course of the transcription reaction and interactions between RNA and the inorganic phase. Previous work demonstrated that polycations can be used to condense RNAi microsponges into nanoparticles capable of efficient transfection with low toxicity. Our new findings suggest that the formation of these nanoparticles is mediated by the gradual dissolution of magnesium pyrophosphate that occurs in the presence of polycations. The simple one-pot approach for assembling RNAi microsponges along with their unique properties could make them useful for RNA-based therapeutics.en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Koch Institute Support (Core) Grant P30-CA14051)en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Center for Cancer Nanotechnology Excellence Grant 5 U54 CA151884-02)en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (Postdoctoral Fellowship)en_US
dc.description.sponsorshipNational Health and Medical Research Council (Australia) (CJ Martin Fellowship)en_US
dc.description.sponsorshipNational Science Foundation (U.S.). Graduate Research Fellowshipen_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/smll.201302676en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleComposite RNAi-Microsponges Form through Self-Assembly of the Organic and Inorganic Products of Transcriptionen_US
dc.title.alternativeRNAi-Microsponges Form through Self-Assembly of the Organic and Inorganic Products of Transcriptionen_US
dc.typeArticleen_US
dc.identifier.citationShopsowitz, Kevin E., Young Hoon Roh, Zhou J. Deng, Stephen W. Morton, and Paula T. Hammond. “RNAi-Microsponges Form through Self-Assembly of the Organic and Inorganic Products of Transcription.” Small 10, no. 8 (December 19, 2013): 1623–1633.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorShopsowitz, Kevinen_US
dc.contributor.mitauthorRoh, Young Hoonen_US
dc.contributor.mitauthorDeng, Zhou J.en_US
dc.contributor.mitauthorMorton, Stephen Winforden_US
dc.contributor.mitauthorHammond, Paula T.en_US
dc.relation.journalSmallen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsShopsowitz, Kevin E.; Roh, Young Hoon; Deng, Zhou J.; Morton, Stephen W.; Hammond, Paula T.en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-3988-0837
mit.licenseOPEN_ACCESS_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record