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dc.contributor.authorLien, Wen-Hui
dc.contributor.authorGuo, Xingyi
dc.contributor.authorPolak, Lisa
dc.contributor.authorLawton, Lee N.
dc.contributor.authorYoung, Richard A.
dc.contributor.authorZheng, Deyou
dc.contributor.authorFuchs, Elaine
dc.date.accessioned2014-12-16T17:08:42Z
dc.date.available2014-12-16T17:08:42Z
dc.date.issued2011-09
dc.date.submitted2011-07
dc.identifier.issn19345909
dc.identifier.urihttp://hdl.handle.net/1721.1/92327
dc.description.abstractUsing mouse skin, where bountiful reservoirs of synchronized hair follicle stem cells (HF-SCs) fuel cycles of regeneration, we explore how adult SCs remodel chromatin in response to activating cues. By profiling global mRNA and chromatin changes in quiescent and activated HF-SCs and their committed, transit-amplifying (TA) progeny, we show that polycomb-group (PcG)-mediated H3K27-trimethylation features prominently in HF-lineage progression by mechanisms distinct from embryonic-SCs. In HF-SCs, PcG represses nonskin lineages and HF differentiation. In TA progeny, nonskin regulators remain PcG-repressed, HF-SC regulators acquire H3K27me3-marks, and HF-lineage regulators lose them. Interestingly, genes poised in embryonic stem cells, active in HF-SCs, and PcG-repressed in TA progeny encode not only key transcription factors, but also signaling regulators. We document their importance in balancing HF-SC quiescence, underscoring the power of chromatin mapping in dissecting SC behavior. Our findings explain how HF-SCs cycle through quiescent and activated states without losing stemness and define roles for PcG-mediated repression in governing a fate switch irreversibly.en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.stem.2011.07.015en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevieren_US
dc.titleGenome-wide Maps of Histone Modifications Unwind In Vivo Chromatin States of the Hair Follicle Lineageen_US
dc.typeArticleen_US
dc.identifier.citationLien, Wen-Hui, Xingyi Guo, Lisa Polak, Lee N. Lawton, Richard A. Young, Deyou Zheng, and Elaine Fuchs. “Genome-Wide Maps of Histone Modifications Unwind In Vivo Chromatin States of the Hair Follicle Lineage.” Cell Stem Cell 9, no. 3 (September 2011): 219–232. © 2011 Elsevier Inc.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorYoung, Richard A.en_US
dc.relation.journalCell Stem Cellen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLien, Wen-Hui; Guo, Xingyi; Polak, Lisa; Lawton, Lee N.; Young, Richard A.; Zheng, Deyou; Fuchs, Elaineen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8855-8647
dspace.mitauthor.errortrue
mit.licensePUBLISHER_POLICYen_US


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