Development and application of control tools for use in optogenetics research
Name
903904509-MIT.pdf
Description
Full printable version
Size
17.58 MB
Format
Adobe PDF
Checksum (MD5)
cb4873cdb0c30f3346fea4d490f35bc8
Author(s)
Monahan, Patrick Erin, III
Advisor(s)
Edward S. Boyden.
Date Issued
2014
Publisher
Massachusetts Institute of Technology
Abstract
Optogenetic actuators such as Channelrhodopsin-2 (ChR2) are seven-transmembrane proteins that function as light-gated ion channels. These naturally occurring proteins are found in green algae and serve as sensory photoreceptors controlling phototaxis. Operationally, they contain the light-isomerizable chromophore all-trans-retinal that, upon absorption of a photon at or around 473nm, a conformational change to 13-cis-retinal is induced. This change opens the channel allowing cations to flow through. In the absence of light, the 13-cis-retinal relaxes back to the resting all-trans-retinal conformation and the channel closes. When an actuators packaged into a lox-containing Adeno-associated virus is used in conjunction with a mouse that expresses the Cre recombinase enzyme in a specific cell type, cell specific expression of the opsin is achieved. When used with LEDs, lasers, or specifically fabricated light delivery tools, control of very specific neural networks is realized. This thesis provides a review of optogenetics and details the development and application of a novel wireless device to optically control neural circuits and behavior.
Description
Thesis: S.M., Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2014.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Subjects
Architecture. Program in Media Arts and Sciences.
MIT Department
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
Persistent DSpace Link
