| dc.contributor.author | Wu, Xuebing | |
| dc.contributor.author | Kriz, Andrea J. | |
| dc.contributor.author | Sharp, Phillip A. | |
| dc.date.accessioned | 2015-04-24T18:55:24Z | |
| dc.date.available | 2015-04-24T18:55:24Z | |
| dc.date.issued | 2014-08 | |
| dc.date.submitted | 2014-06 | |
| dc.identifier.issn | 2095-4689 | |
| dc.identifier.issn | 2095-4697 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/96803 | |
| dc.description.abstract | The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (United States Public Health Service Grant RO1-GM34277) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (United States Public Health Service Grant R01-CA133404) | en_US |
| dc.description.sponsorship | National Cancer Institute (U.S.) (Grant PO1-CA42063) | en_US |
| dc.description.sponsorship | National Cancer Institute (U.S.) (Koch Institute Support (Core) Grant P30-CA14051) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Springer-Verlag | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1007/s40484-014-0030-x | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | PMC | en_US |
| dc.title | Target specificity of the CRISPR-Cas9 system | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Wu, Xuebing, Andrea J. Kriz, and Phillip A. Sharp. “Target Specificity of the CRISPR-Cas9 System.” Quantitative Biology 2, no. 2 (June 2014): 59–70. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
| dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
| dc.contributor.mitauthor | Wu, Xuebing | en_US |
| dc.contributor.mitauthor | Kriz, Andrea J. | en_US |
| dc.contributor.mitauthor | Sharp, Phillip A. | en_US |
| dc.relation.journal | Quantitative Biology | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Wu, Xuebing; Kriz, Andrea J.; Sharp, Phillip A. | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0003-1465-1691 | |
| dc.identifier.orcid | https://orcid.org/0000-0003-0369-5269 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
| mit.metadata.status | Complete | |
