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A rapid survival assay to measure drug-induced cytotoxicity and cell cycle effects

Author(s)
Valiathan, Chandni; McFaline, Jose Luis; Samson, Leona D.
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Abstract
We describe a rapid method to accurately measure the cytotoxicity of mammalian cells upon exposure to various drugs. Using this assay, we obtain survival data in a fraction of the time required to perform the traditional clonogenic survival assay, considered the gold standard. The dynamic range of the assay allows sensitivity measurements on a multi-log scale allowing better resolution of comparative sensitivities. Moreover, the results obtained contain additional information on cell cycle effects of the drug treatment. Cell survival is obtained from a quantitative comparison of proliferation between drug-treated and untreated cells. During the assay, cells are treated with a drug and, following a recovery period, allowed to proliferate in the presence of bromodeoxyuridine (BrdU). Cells that synthesize DNA in the presence of BrdU exhibit quenched Hoechst fluorescence, easily detected by flow cytometry; quenching is used to determine relative proliferation in treated vs. untreated cells. Finally, this assay can be used in high-throughput format to simultaneously screen multiple cell lines and drugs for accurate measurements of cell survival and cell cycle effects after drug treatment.
Date issued
2011-11
URI
http://hdl.handle.net/1721.1/99508
Department
Massachusetts Institute of Technology. Center for Environmental Health Sciences; Massachusetts Institute of Technology. Computational and Systems Biology Program; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal
DNA Repair
Publisher
Elsevier
Citation
Valiathan, Chandni, Jose L. McFaline, and Leona D. Samson. “A Rapid Survival Assay to Measure Drug-Induced Cytotoxicity and Cell Cycle Effects.” DNA Repair 11, no. 1 (January 2012): 92–98.
Version: Author's final manuscript
ISSN
15687864

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