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dc.contributor.advisorKatharina Ribbeck.en_US
dc.contributor.authorCo, Julia Yin-Tingen_US
dc.contributor.otherMassachusetts Institute of Technology. Department of Biology.en_US
dc.date.accessioned2015-10-30T18:56:19Z
dc.date.available2015-10-30T18:56:19Z
dc.date.copyright2015en_US
dc.date.issued2015en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/99569
dc.descriptionThesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2015.en_US
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.description.abstractMucus is the hydrogel layer that coats all wet epithelia in the body. By supporting commensal microbes and preventing pathogenic invasion, mucus maintains host-microbe homeostasis. Mucin polymers, the primary gel-forming component of mucus, are an important mediator of mucus-microbe interactions. In this thesis, I demonstrate that mucins impact bacterial communities in their physical structure as well as microbe-microbe and microbe-host dynamics. In Chapter 2, 1 study the ability of mucin surface coatings to suppress bacterial surface attachment, the first step in biofilm formation, for Streptococcus pneumoniae and Staphylococcus aureus. Mucin-bound glycans were identified as a critical structural component of mucin coatings that are necessary for bacterial repulsion. In Chapter 3, 1 investigate how mucins impact established Pseudomonas aeruginosa biofilms. The data reveal that mucins cause disassembly and structural rearrangement in P. aeruginosa biofilms in a mucin concentration and flow rate dependent manner. In Appendix A, I show evidence for the involvement of the bacterial flagella in mucinmediated biofilm disruption. Deletion of flagellar capfliD or flagellar stators motABCD results in biofilms that are resistant to mucin-mediated dissociation. In Appendix B, I examine how mucins affect dual-species bacterial communities. I show that mucins promote S. aureus survival during co-culture with P. aeruginosa and also suppress the anti-staphylococcal effects of P. aeruginosa pyocyanin. In Appendix C, I explore the impacts of mucins on P. aeruginosa quorum sensing, an important pathogenic determinant in P. aeruginosa infections. I found that mucins suppress the expression of P. aeruginosa Las and Rhl quorum sensing genes as well as downstream virulence factors. In Appendix D, I assess how mucins modulate P. aeruginosa-epithelium interactions. The data show that mucins hinder the ability of P. aeruginosa to attach to epithelial cells in vitro. Additionally, mucins suppressed P. aeruginosa-associatede pithelial cytotoxicity in a mucin concentration dependent manner. Together, this thesis demonstrates that mucins modulate microbial communities in their behavior and interactions. Understanding how mucus and mucins impact microbes provides insight to host-microbe relationships, as well as for the development of novel bacteria-regulating strategies.en_US
dc.description.statementofresponsibilityby Julia Yin-Ting Co.en_US
dc.format.extent102 pagesen_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsM.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectBiology.en_US
dc.titleThe influence of mucins on bacterial communitiesen_US
dc.typeThesisen_US
dc.description.degreePh. D.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biology
dc.identifier.oclc924780500en_US


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