2026-09-18T20:31:07Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1088792022-01-13T07:53:55Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Peter Reddien.
Owen, Jared H. (Jared Huntsman)
Massachusetts Institute of Technology. Department of Biology.
Massachusetts Institute of Technology. Department of Biology
2017-05-11T19:53:29Z
2017-05-11T19:53:29Z
2017
2017
http://hdl.handle.net/1721.1/108879
986239543
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2017.
Cataloged from PDF version of thesis. "t" at beginning of title is letter "t" in lower case.
Includes bibliographical references (pages 28-33).
Regeneration requires that the identities of new cells are properly specified to replace missing tissues. The Wnt signaling pathway serves a central role in specifying posterior cell fates during planarian regeneration. We identified a gene encoding a homolog of the Teashirt family of zinc finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt signaling in intact animals and at posterior-facing wounds. Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes [beta]-catenin-1, wntl, Dvl-1/2, or wntless. teashirt was required for [beta]-catenin-I-dependent activation of posterior genes during regeneration. These findings identify teashirt as a transcriptional target of Wnt signaling required for Wnt-mediated specification of posterior blastemas.
by Jared H. Owen.
S.M.
59 pages
eng
Massachusetts Institute of Technology
MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.
http://dspace.mit.edu/handle/1721.1/7582
Biology.
teashirt is required for head-versus-tail regeneration polarity in planarians
Thesis
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<Abstract>Regeneration requires that the identities of new cells are properly specified to replace missing tissues. The Wnt signaling pathway serves a central role in specifying posterior cell fates during planarian regeneration. We identified a gene encoding a homolog of the Teashirt family of zinc finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt signaling in intact animals and at posterior-facing wounds. Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes [beta]-catenin-1, wntl, Dvl-1/2, or wntless. teashirt was required for [beta]-catenin-I-dependent activation of posterior genes during regeneration. These findings identify teashirt as a transcriptional target of Wnt signaling required for Wnt-mediated specification of posterior blastemas.</Abstract>
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