2026-09-19T11:19:21Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1143302022-01-13T07:53:59Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Roger Summons.
Dibbell, Augusta K
Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences.
Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences
2018-03-27T14:17:47Z
2018-03-27T14:17:47Z
2007
2007
http://hdl.handle.net/1721.1/114330
1028748586
Thesis: S.B., Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences, 2007.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 49-50).
Stabe isotope probe (SIP) incubation studies were performed using ¹³C-labeled carbon substrates on hyperthermophilic filamentous streamer communities inhabiting Octopus Spring in Yellowstone National Park. Biomass was removed from the outflow stream and incubated at near-in situ conditions with labeled bicarbonate, formate, or acetate. Lipids from the biomass were extracted and analyzed using gas chromatography-mass spectroscopy (GC-MS) and gas chromatography-isotope ratio monitoring-mass spectroscopy(GC-IRMS). We observed incorporation of ¹³C-labeled acetate into the total biomass, archaeal lipids, and a small number of bacterial lipids, but no incorporation of labeled formate or bicarbonate. During 67 hours of incubation, 0.060 [mu]g of labeled acetate was incorporated by the archaeal and bacterial community. The lack of acetate incorporation by most bacteria, or formate and bicarbonate incorporation by any community member may reflect rates of carbon turnover, the carbon acquisition pathway used, or inhibition under experimental conditions,
by Augusta K. Dibbell.
S.B.
50 pages
eng
Massachusetts Institute of Technology
MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.
http://dspace.mit.edu/handle/1721.1/7582
Earth, Atmospheric, and Planetary Sciences.
Stable isotope probing of hyperthermophilic filamentous microbial communities in Octopus Spring, Yellowstone National Park
Thesis
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Publication
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<Abstract>Stabe isotope probe (SIP) incubation studies were performed using ¹³C-labeled carbon substrates on hyperthermophilic filamentous streamer communities inhabiting Octopus Spring in Yellowstone National Park. Biomass was removed from the outflow stream and incubated at near-in situ conditions with labeled bicarbonate, formate, or acetate. Lipids from the biomass were extracted and analyzed using gas chromatography-mass spectroscopy (GC-MS) and gas chromatography-isotope ratio monitoring-mass spectroscopy(GC-IRMS). We observed incorporation of ¹³C-labeled acetate into the total biomass, archaeal lipids, and a small number of bacterial lipids, but no incorporation of labeled formate or bicarbonate. During 67 hours of incubation, 0.060 [mu]g of labeled acetate was incorporated by the archaeal and bacterial community. The lack of acetate incorporation by most bacteria, or formate and bicarbonate incorporation by any community member may reflect rates of carbon turnover, the carbon acquisition pathway used, or inhibition under experimental conditions,</Abstract>
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