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Jeffrey A. Hoffman. Bhattaru, Srinivasa Aditya Massachusetts Institute of Technology. Department of Aeronautics and Astronautics. Massachusetts Institute of Technology. Department of Aeronautics and Astronautics 2018-11-15T16:34:53Z 2018-11-15T16:34:53Z 2018 2018 http://hdl.handle.net/1721.1/119060 1057726335 Thesis: S.M., Massachusetts Institute of Technology, Department of Aeronautics and Astronautics, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (pages 75-78). The development of any spaceflight instrument involves a systematic, iterative process of design, testing, and validation. This ensures that the system developed will meet the needs of stakeholders while minimizing costs and risks. Here, the needs for a life-detection instrument targeting nucleic acids are identified, the objectives for that instrument are determined, and system engineering analysis is used to demonstrate that a Search for Extra-Terrestrial Genomes instrument would fulfill those objectives and is feasible for a rover mission. Additionally, we show our design and build process for a testbed to rapidly prototype SETG components and subsystems, which has successfully automated nucleic acid extraction, sequencer loading, and parts of library preparation. We also experiment with thermal simulations and conduct a sequencing test at Martian conditions, using a custom built thermal vacuum chamber system. Finally, this thesis explores potential avenues for future development and identifies short term and long term engineering goals that would assist the SETG team in developing an instrument prototype. by Srinivasa Aditya Bhattaru. S.M. 78 pages eng Massachusetts Institute of Technology MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 Aeronautics and Astronautics. Design, testing, and validation of the search for Extra-Terrestrial Genomes instrument Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="5e0f83de-f978-4f29-b934-cd99a0b479c4"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Design, testing, and validation of the search for Extra-Terrestrial Genomes instrument</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2018</PublicationDate> <Authors> <Author> <DisplayName>Bhattaru, Srinivasa Aditya</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Aeronautics and Astronautics.</Keyword> <Abstract>The development of any spaceflight instrument involves a systematic, iterative process of design, testing, and validation. This ensures that the system developed will meet the needs of stakeholders while minimizing costs and risks. Here, the needs for a life-detection instrument targeting nucleic acids are identified, the objectives for that instrument are determined, and system engineering analysis is used to demonstrate that a Search for Extra-Terrestrial Genomes instrument would fulfill those objectives and is feasible for a rover mission. Additionally, we show our design and build process for a testbed to rapidly prototype SETG components and subsystems, which has successfully automated nucleic acid extraction, sequencer loading, and parts of library preparation. We also experiment with thermal simulations and conduct a sequencing test at Martian conditions, using a custom built thermal vacuum chamber system. Finally, this thesis explores potential avenues for future development and identifies short term and long term engineering goals that would assist the SETG team in developing an instrument prototype.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>