2026-09-19T14:25:19Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1400732022-02-08T03:30:52Zcom_1721.1_7582com_1721.1_7581col_1721.1_131022
Han, Jongyoon
Choi, Kyungyong
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
2022-02-07T15:22:29Z
2022-02-07T15:22:29Z
2021-09
2021-09-21T19:30:43.295Z
https://hdl.handle.net/1721.1/140073
https://orcid.org/0000-0003-0935-4158
Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing.
Ph.D.
Massachusetts Institute of Technology
In Copyright - Educational Use Permitted
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http://rightsstatements.org/page/InC-EDU/1.0/
Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
Thesis
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Doctoral
Doctor of Philosophy
Publication
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<Abstract>Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing.</Abstract>
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