2026-09-20T04:43:13Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1466772022-12-01T03:16:29Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
David L. Trumper
Dey Barsukova, Anita
Massachusetts Institute of Technology. Department of Mechanical Engineering
2022-11-30T19:40:48Z
2022-11-30T19:40:48Z
2022-05
2022-08-29T20:43:51.172Z
https://hdl.handle.net/1721.1/146677
Microphysiological systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or "Oxychip", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated.
S.B.
Massachusetts Institute of Technology
In Copyright - Educational Use Permitted
Copyright MIT
http://rightsstatements.org/page/InC-EDU/1.0/
Integrated Microfluidic Culture Media Oxygenator for Organ-on-a-Chip Applications
Thesis
application/pdf
Bachelor
Bachelor of Science in Mechanical Engineering
Publication
unknown
unknown
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<Abstract>Microphysiological systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or "Oxychip", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated.</Abstract>
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