2026-09-22T02:02:44Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1484222026-06-16T18:17:40Zcom_1721.1_7582com_1721.1_7581col_1721.1_131022
Gerald N. Wogan.
Thilly, William George.
Massachusetts Institute of Technology. Department of Nutrition and Food Science.
Massachusetts Institute of Technology. Department of Nutrition and Food Science
2023-03-08T20:24:54Z
2023-03-08T20:24:54Z
1971
https://hdl.handle.net/1721.1/148422
1371248901
Thesis: Sc. D., Massachusetts Institute of Technology, Department of Nutrition and Food Science, 1971
Includes bibliographical references (pages 211-215, 216a-216g).
HeLa S3 cells have been induced to divide synchronously in suspension culture through more than ten generations. Cellular DNA and RNA have been determined at hourly intervals throughout the cell cycle defining the pattern of nucleic acid accumulation in HeLa cells. Cellular activity of the enzymes thymidine kinase, alkaline DNase, catalase, acid phosphatase and lactic dehydrogenase has been determined as a function of the division cycle. The method of synchronization developed here allows the continuous synchronous growth of mammalian cells in suspension culture. This system facilitates studies of cell cycle events since it provides a large amount of cellular material with minimal maintenance time required.
by William George Thilly.
Sc.D.
Sc. D. Massachusetts Institute of Technology, Department of Nutrition and Food Science
xi, 261 pages
eng
Massachusetts Institute of Technology
MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.
http://dspace.mit.edu/handle/1721.1/7582
Nutrition and Food Science.
Studies of the division cycle of human cancer cells (HELA) using a new system for continuous synchronization
Thesis
application/pdf
2023-03-08T20:24:54Z
Publication
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<Abstract>HeLa S3 cells have been induced to divide synchronously in suspension culture through more than ten generations. Cellular DNA and RNA have been determined at hourly intervals throughout the cell cycle defining the pattern of nucleic acid accumulation in HeLa cells. Cellular activity of the enzymes thymidine kinase, alkaline DNase, catalase, acid phosphatase and lactic dehydrogenase has been determined as a function of the division cycle. The method of synchronization developed here allows the continuous synchronous growth of mammalian cells in suspension culture. This system facilitates studies of cell cycle events since it provides a large amount of cellular material with minimal maintenance time required.</Abstract>
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