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Jacobson, Joseph Calman, Ido Program in Media Arts and Sciences (Massachusetts Institute of Technology) 2023-08-30T15:58:40Z 2023-08-30T15:58:40Z 2023-06 2023-08-16T20:34:00.537Z https://hdl.handle.net/1721.1/152003 https://orcid.org/0009-0007-4177-4415 Protein-DNA interactions play a critical role in various biological processes, such as gene regulation and genome maintenance. Designing protein backbones specifically tailored for DNA binding remains a challenging task, requiring the exploration of novel computational approaches. This thesis presents a novel framework for gen- erating protein backbones that exhibit affinity for DNA molecules. The proposed methodology leverages Graph Neural Networks (GNNs) for encoding protein struc- tures and diffusion models for conditional sampling. The GNNs capture the intricate relationships between amino acids in the protein backbone, allowing for the effective encoding of structural information relevant to DNA binding. The diffusion models enable the conditional generation of protein backbones, given specific DNA sequences as input. The thesis proposes a Transformer architecture and provides a practical way to diffuse from its protein encoding. The findings from this research have significant implications for the design and engineering of DNA binding proteins, facilitating ad- vancements in fields such as synthetic biology, gene therapy, and drug development. S.M. Massachusetts Institute of Technology In Copyright - Educational Use Permitted Copyright retained by author(s) https://rightsstatements.org/page/InC-EDU/1.0/ Designing Novel DNA-Binding Proteins with Generative Deep Learning Thesis application/pdf Master Master of Science in Media Arts and Sciences Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="c53f2cb5-e77e-4c6c-92d7-9cc4d362d9c5"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Title>Designing Novel DNA-Binding Proteins with Generative Deep Learning</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2023-06</PublicationDate> <Authors> <Author> <DisplayName>Calman, Ido</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://rightsstatements.org/page/InC-EDU/1.0/</License> <Abstract>Protein-DNA interactions play a critical role in various biological processes, such as gene regulation and genome maintenance. Designing protein backbones specifically tailored for DNA binding remains a challenging task, requiring the exploration of novel computational approaches. This thesis presents a novel framework for gen- erating protein backbones that exhibit affinity for DNA molecules. The proposed methodology leverages Graph Neural Networks (GNNs) for encoding protein struc- tures and diffusion models for conditional sampling. The GNNs capture the intricate relationships between amino acids in the protein backbone, allowing for the effective encoding of structural information relevant to DNA binding. The diffusion models enable the conditional generation of protein backbones, given specific DNA sequences as input. The thesis proposes a Transformer architecture and provides a practical way to diffuse from its protein encoding. The findings from this research have significant implications for the design and engineering of DNA binding proteins, facilitating ad- vancements in fields such as synthetic biology, gene therapy, and drug development.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>