2026-09-19T22:18:52Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1528242023-11-03T03:54:13Zcom_1721.1_7582com_1721.1_7581col_1721.1_131022
Zhang, Feng
Regev, Aviv
Frangieh, Chris J.
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
2023-11-02T20:19:08Z
2023-11-02T20:19:08Z
2023-09
2023-09-21T14:26:27.029Z
https://hdl.handle.net/1721.1/152824
Genomes are the basis of human biology and human disease. Understanding the role of each gene on a healthy or diseased phenotype requires an intervention to causally link between genotype and phenotype. Advances in RNA-guided endonucleases have enabled such pooled screens in human cells. I first consider a model to understand drivers of immune evasion in a pooled knockout screen conducted in an in vitro model of metastatic melanoma. Next, I discuss strategies for scaling these screens to encompass a larger set of genes from the human genome. Finally, I explore how next-generation genome editors can move beyond knockout screens to identify the biological role of any sequence at any location in the human genome.
Ph.D.
Massachusetts Institute of Technology
In Copyright - Educational Use Permitted
Copyright retained by author(s)
https://rightsstatements.org/page/InC-EDU/1.0/
Methods and models of screening genomic variants
Thesis
application/pdf
Doctoral
Doctor of Philosophy
Publication
unknown
unknown
<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="340dc36e-dbcb-42c4-98d1-ec59daea118d">
<Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type>
<Title>Methods and models of screening genomic variants</Title>
<PublishedIn>
<Publication>
</Publication>
</PublishedIn>
<PublicationDate>2023-09</PublicationDate>
<Authors>
<Author>
<DisplayName>Frangieh, Chris J.</DisplayName>
<Affiliation>
<OrgUnit>
</OrgUnit>
</Affiliation>
</Author>
</Authors>
<Editors>
</Editors>
<Publishers>
<Publisher>
<DisplayName>Massachusetts Institute of Technology</DisplayName>
<OrgUnit />
</Publisher>
</Publishers>
<License>https://rightsstatements.org/page/InC-EDU/1.0/</License>
<Abstract>Genomes are the basis of human biology and human disease. Understanding the role of each gene on a healthy or diseased phenotype requires an intervention to causally link between genotype and phenotype. Advances in RNA-guided endonucleases have enabled such pooled screens in human cells. I first consider a model to understand drivers of immune evasion in a pooled knockout screen conducted in an in vitro model of metastatic melanoma. Next, I discuss strategies for scaling these screens to encompass a larger set of genes from the human genome. Finally, I explore how next-generation genome editors can move beyond knockout screens to identify the biological role of any sequence at any location in the human genome.</Abstract>
<Access xmlns="http://purl.org/coar/access_right"
>
</Access>
</Publication>