2026-09-18T22:20:06Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/1541222025-10-30T15:50:05Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Edward S. Boyden.
Johnson, Shannon L.
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
2024-04-10T21:40:47Z
2024-04-10T21:40:47Z
2019
2019
https://hdl.handle.net/1721.1/154122
1418760885
Thesis: S.M., Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2019
Cataloged from the official PDF version of thesis.
Includes bibliographical references (pages 38-40).
To circumvent the limitations of spectrally multiplexing sensors, fluorescent sensors are clustered by type and spatially separated in the cytoplasm to avoid cross-talk. Each sensor is fused to an orthogonal viral capsid protein that binds to a long, repetitive strand of its corresponding RNA sequence. All sensors fluoresce green and are indistinguishable during recording but are identified with post-hoc antibody or FISH staining for each sensor-specific puncta. This spatial multiplexing strategy will allow for easier scaling of the number of fluorescent reporters of physiological activity.
by Shannon L. Johnson.
S.M.
S.M. Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences
54 pages
eng
Massachusetts Institute of Technology
MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.
http://dspace.mit.edu/handle/1721.1/7582
Program in Media Arts and Sciences
Intracellular sensor spatial multiplexing via RNA scaffolds
Thesis
application/pdf
2024-04-10T21:40:47Z
Publication
Master
unknown
unknown
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