2026-09-19T06:47:35Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/282862022-01-13T07:54:33Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Darrell J. Irvine.
Hong, Julee Y. (Julee Yang-A.), 1980-
Massachusetts Institute of Technology. Dept. of Materials Science and Engineering.
Massachusetts Institute of Technology. Department of Materials Science and Engineering
2005-09-26T19:34:50Z
2005-09-26T19:34:50Z
2003
2003
http://hdl.handle.net/1721.1/28286
54524845
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2003.
Includes bibliographical references (leaf 29).
The feasibility of bringing a nascent technology for detection and quantification of local cytokine concentrations on cell surfaces to market is presented in this paper. Quantum dots or fluorochrome-loaded nanoparticles are conjugated with antibodies for target analytes and with proteins that allow nanoparticle attachment to the surface of T cells. A second labeled monoclonal antibody is introduced to detect the presence of any captured-cytokines using 3D fluorescent microscopy or flow cytometry. Microscopy of DO.11 cells labeled with cytokinecapture particles have shown successful detection of exogenous IL2. A comparison of existing patents with cytokine-capture technology revealed that although each aspect of the device is covered by prior IP, the capabilities of the technology exceed the claimed uses of the individual components. A preliminary market research for cytokine-capture technology applications resulted in dismissing the immunoassay industry as a target market. However, T cell monitoring was identified as a far more lucrative industry.
by Julee Y. Hong.
S.B.
29 leaves
3997127 bytes
4003410 bytes
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en_US
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Materials Science and Engineering.
Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation
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