2026-09-19T14:40:53Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/286232022-01-13T07:53:48Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Scott R. Manalis.
Levy-Tzedek, Shelly
Massachusetts Institute of Technology. Biological Engineering Division.
Massachusetts Institute of Technology. Department of Biological Engineering
2005-09-27T17:22:23Z
2005-09-27T17:22:23Z
2004
2004
http://hdl.handle.net/1721.1/28623
57569643
Thesis (S.M.)--Massachusetts Institute of Technology, Biological Engineering Division, 2004.
Includes bibliographical references (p. 33-37).
Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel resonator offers the opportunity to perform such label-free measurements. The goal of this work is to open new avenues of possible applications for the suspended channel. I introduce the concept of detecting mass subtraction as a new approach, rather than the conventional detection of mass addition. In a model implementation scenario of this approach, a mass-intensifying tag bound to a small ligand molecule will be equilibrated with surface-immobilized receptors, and later displaced by an identical, but label-free, ligand molecule. This approach offers opportunities to extend the sensitivity range of the device, as well as introduces new functionality for it. It enables researchers to follow, label-free, real-time enzymatic reactions, relative affinities of different ligands to a receptor, and presence of small molecules in a solution.
by Shelly Levy-Tzedek.
S.M.
37 p.
2843473 bytes
2845328 bytes
application/pdf
application/pdf
en_US
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Biological Engineering Division.
Biological detection by means of mass reduction in a suspended microchannel resonator
Thesis
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