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Harold F. Hemond and David B. Schauer. Jellison, Kristen L. (Kristen Leigh), 1975- Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering. Massachusetts Institute of Technology. Department of Civil and Environmental Engineering 2005-10-14T20:04:04Z 2005-10-14T20:04:04Z 2003 2003 http://hdl.handle.net/1721.1/29359 52769942 Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2003. Includes bibliographical references. Cryptosporidium parvum is responsible for an acute gastrointestinal disease that is self-limiting in immunocompetent people but potentially life-threatening for the immunocompromised. Until recently, C. parvum was the only species of Cryptosporidium known to cause disease in people, however, reports of C. muris, C. felis, and C. meleagridis in immunocompetent adults have raised questions about the extent to which Cryptosporidium spp. are infectious for humans. Until more is known, presence of any Cryptosporidium oocysts in the environment should be considered a potential public health risk. Cryptosporidium spp. can infect a wide range of animal hosts, and environmental sources may include wildlife, agricultural animals, or human sewage. Transmission of Cryptosporidium spp. via fecally-contaminated food and water has been well-documented, and outbreaks of cryptosporidiosis have occurred around the world. The exogenous stage of the organism, the oocyst, is difficult to remove from drinking water supplies because it is resistant to chlorine disinfection and inefficiently filtered. Therefore, a better understanding of the sources, fate, and transport of oocysts in the environment is critical to protect source waters from oocyst contamination. In this work, a sensitive and specific molecular detection assay for Cryptosporidium spp. in environmental samples was developed and applied to surface water and fecal samples from the Wachusett Reservoir watershed, the drinking water source for metropolitan Boston, to establish links between oocyst sources and surface water contamination. Multiple species of Cryptosporidium were detected, and previously uncharacterized genetic diversity at the 18S rRNA locus was observed. (cont.) Each surface water site had a hypothesized oocyst source, but results showed that the sources detected were often very different from those hypothesized to be most important. Cryptosporidium spp. from wildlife was detected in surface waters hypothesized to be contaminated by human sewage, and surface waters susceptible to agricultural runoff were observed to be more impacted by birds. In addition, Cryptosporidium spp. contamination occurred seasonally, with the seasonal pattern of detection distinct for surface waters with different oocyst sources. Results of this work contribute to a growing characterization of Cryptosporidium in the environment that will ultimately help minimize public exposure to this waterborne parasite. by Kristen L. Jellison. Ph.D. 233 p. 8467708 bytes 8467517 bytes application/pdf application/pdf eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Civil and Environmental Engineering. Molecular and genetic analysis of Cryptosporidium spp. oocysts : sources and genotypes in the environment Thesis application/pdf false Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="bc2a42e8-094f-4dac-b6f3-ab4aa16e1f66"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Molecular and genetic analysis of Cryptosporidium spp. oocysts : sources and genotypes in the environment</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2003</PublicationDate> <Authors> <Author> <DisplayName>Jellison, Kristen L. (Kristen Leigh), 1975-</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Civil and Environmental Engineering.</Keyword> <Abstract>Cryptosporidium parvum is responsible for an acute gastrointestinal disease that is self-limiting in immunocompetent people but potentially life-threatening for the immunocompromised. Until recently, C. parvum was the only species of Cryptosporidium known to cause disease in people, however, reports of C. muris, C. felis, and C. meleagridis in immunocompetent adults have raised questions about the extent to which Cryptosporidium spp. are infectious for humans. Until more is known, presence of any Cryptosporidium oocysts in the environment should be considered a potential public health risk. Cryptosporidium spp. can infect a wide range of animal hosts, and environmental sources may include wildlife, agricultural animals, or human sewage. Transmission of Cryptosporidium spp. via fecally-contaminated food and water has been well-documented, and outbreaks of cryptosporidiosis have occurred around the world. The exogenous stage of the organism, the oocyst, is difficult to remove from drinking water supplies because it is resistant to chlorine disinfection and inefficiently filtered. Therefore, a better understanding of the sources, fate, and transport of oocysts in the environment is critical to protect source waters from oocyst contamination. In this work, a sensitive and specific molecular detection assay for Cryptosporidium spp. in environmental samples was developed and applied to surface water and fecal samples from the Wachusett Reservoir watershed, the drinking water source for metropolitan Boston, to establish links between oocyst sources and surface water contamination. Multiple species of Cryptosporidium were detected, and previously uncharacterized genetic diversity at the 18S rRNA locus was observed.</Abstract> <Abstract>(cont.) Each surface water site had a hypothesized oocyst source, but results showed that the sources detected were often very different from those hypothesized to be most important. Cryptosporidium spp. from wildlife was detected in surface waters hypothesized to be contaminated by human sewage, and surface waters susceptible to agricultural runoff were observed to be more impacted by birds. In addition, Cryptosporidium spp. contamination occurred seasonally, with the seasonal pattern of detection distinct for surface waters with different oocyst sources. Results of this work contribute to a growing characterization of Cryptosporidium in the environment that will ultimately help minimize public exposure to this waterborne parasite.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>