2026-09-20T14:10:05Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/294452022-01-13T07:54:19Zcom_1721.1_7582com_1721.1_7581col_1721.1_131022
Douglas A. Lauffenburger and Peter K. Sorger.
Duggar, Keith Howard, 1976-
Massachusetts Institute of Technology. Dept. of Chemical Engineering.
Massachusetts Institute of Technology. Department of Chemical Engineering
2005-10-14T20:35:39Z
2005-10-14T20:35:39Z
2004
2004
http://hdl.handle.net/1721.1/29445
56205129
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2004.
Includes bibliographical references (p. 65-66).
Gene expression arrays are a technology used to measure quantities of messenger ribonucleic acid (mRNA). Application of the technology involves a variety of physical processes beginning with the acquisition of mRNA samples and ending with the fluorescence imaging of a gene expression array. This thesis examines these physical processes, develops a mechanistic model, and derives the analysis procedure based on the model. Chief advantages of this approach are that it accounts for certain previously unexplained array phenomena and is based in a clear way on physical knowledge allowing non-arbitrary determination of both the probability that any given gene has altered expression ratio relative to a control as well as the magnitude of this induction or repression. We demonstrate its use on simulated and real array data, and show that a considerable amount of previously unrecognized information concerning gene expression differences is inherent in the array measurements.
by Keith Howard Duggar.
Ph.D.
66 p.
2133621 bytes
2133429 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Chemical Engineering.
Modeling and analysis of gene expression arrays
Thesis
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