2026-09-20T00:26:04Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/327892022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Matthew J. Lang.
Ta, Jenny, 1982-
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2006-05-15T20:30:14Z
2006-05-15T20:30:14Z
2004
2004
http://hdl.handle.net/1721.1/32789
57583131
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
Includes bibliographical references (p. 37).
The recent development in combined optical trapping and fluorescence technology promises to enable unbindinig force studies of receptor-ligand interactions, whose specificity play a crucial role in the function of many biological systems. This thesis focuses on the development of assay designs for the study of antibody-antigen binding interactions using combined optical trapping and single molecule fluorescence. The assays create the necessary linkage geometry between the antibody-antigen system under study to an optically trapped bead, enabling force probing of the antibody-antigen binding interaction. In particular, two tether materials and fluorophores were studied: polyethylene glycol (PEG) with Cy-2, and dsDNA with fluorescein. We demonstrate tether formation in the dsDNA-fluorescein antibody-antigen linkage system with preliminary optical trapping data.
by Jenny Ta.
S.B.
37 p.
1177088 bytes
1176599 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
Antibody-Antigen assay design for combined optical tweezers and fluorescence
Thesis
application/pdf
Publication
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